Bioreduction performances and mechanisms of Cr(VI) by Sporosarcina saromensis W5, a novel Cr(VI)-reducing facultative anaerobic bacteria.

This study reported a novel facultative anaerobic Cr(VI)-reducing bacteria (Sporosarcina saromensis W5) and investigated its Cr(VI) removal performances and removal mechanisms. The strain W5 was able to grow and reduce Cr(VI) under aerobic and anaerobic environment, and exhibited considerable Cr(VI) reduction capabilities under a wide range of pH (8.0-13.0), temperature (20-40 °C) and initial Cr(VI) concentration (50-800 mg/L). The addition of Cd severely inhibited its growth and Cr(VI) removal, while Cu and Fe significantly enhanced the removal efficiencies. The strain W5 could utilize various electron donors and mediators to accelerate Cr(VI) reduction. Aerobic Cr(VI) reduction mainly occurred in cytoplasm and the final products were soluble organo-Cr(III) complexes. Anaerobic Cr(VI) reduction was located in both cytoplasm and membrane, and the reduction products were soluble organo-Cr(III) complexes and Cr(III) precipitates. The functional groups of hydroxyl, carboxyl and phosphoryl on cell surface participated in the combination with Cr(III). Due to its facultative anaerobic property, S. saromensis W5 offers itself as a promising engineering strain for the bioremediation of Cr(VI)-contaminated areas, especially in hypoxia environments.