Samad Noreen, Bilal Kainat, Yasmin Farzana, Khaliq Saima, Zaman Aqal, Ayaz M Mazhar
Department of Biochemistry, Faculty of Science, Bahauddin Zakariya University, Multan, Pakistan.
Department of Food Engineering, NED University of Engineering and Technology, Karachi, Pakistan/Department of Biomedical Engineering, NED University of Engineering and Technology, Karachi, Pakistan.
Pak J Pharm Sci. 2020 Jul;33(4(Supplementary)):1795-1803.
The aging process is concerned with oxidative stress and causing malfunction of various organs such as the liver, kidney and heart. Lithium (Li) salts have shown anti-manic, anti-suicidal, and antioxidant properties. The current study is aimed to evaluate the possible inhibitory effects of various doses (10, 20 & 40mg/ml/kg) of Lithium chloride (LiCl) on D-galactose (D-gal)-produced aging model and explore the underlying mechanism. In the study 40 male rats were randomly alienated into 8 groups i.e. saline, LiCl (10, 20 & 40mg/ml/kg), D-gal and D-gal+LiCl (10, 20 & 40 mg/ml/kg). D-gal was given at a dosage of 300mg/ml/kg$ and animals received their respective treatment for 6 weeks [intraperitoneally (I.P), once daily]. After 2 weeks animals were decapitated and organs (liver, kidney, and heart) were removed for antioxidant assays. Blood was also collected for biochemical parameters. LiCl substantially decreased oxidative strain marker and increased enzymatic antioxidants in the liver, kidney, and heart of D-gal treated rats. LiCl also decreased serum alanine aminotransferase (ALT), aspartate transaminase (AST), creatine, urea, CK-MB, triglyceride, cholesterol, low-density lipoprotein (LDL) and increased high-density lipoprotein (HDL) in D-gal treated animals. High dose (80mg/ml/kg) of LiCl observed as the most effective dose against D-gal induced alterations. These finding LiCl inhibits D-gal induced liver, kidney and heart damages via its antioxidant potential.
衰老过程与氧化应激有关，并会导致肝脏、肾脏和心脏等各种器官功能失调。锂盐已显示出抗躁狂、抗自杀和抗氧化特性。本研究旨在评估不同剂量（10、20和40mg/ml/kg）的氯化锂（LiCl）对D-半乳糖（D-gal）诱导的衰老模型的可能抑制作用，并探讨其潜在机制。在该研究中，40只雄性大鼠被随机分为8组，即生理盐水组、LiCl（10、20和40mg/ml/kg）组、D-gal组和D-gal + LiCl（10、20和40mg/ml/kg）组。D-gal的给药剂量为300mg/ml/kg，动物接受各自的治疗6周[腹腔注射（I.P.），每日一次]。2周后将动物断头，取出器官（肝脏、肾脏和心脏）进行抗氧化测定。还采集血液检测生化参数。LiCl显著降低了D-gal处理大鼠肝脏、肾脏和心脏中的氧化应激标志物，并增加了酶促抗氧化剂。LiCl还降低了D-gal处理动物血清中的丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、肌酸、尿素、肌酸激酶同工酶（CK-MB）、甘油三酯、胆固醇、低密度脂蛋白（LDL），并增加了高密度脂蛋白（HDL）。高剂量（80mg/ml/kg）的LiCl被认为是对抗D-gal诱导变化的最有效剂量。这些结果表明，LiCl通过其抗氧化潜力抑制D-gal诱导的肝脏、肾脏和心脏损伤。
