Department of Zoology, University of Peshawar Khyber Pakhtunkwa Pakistan.
Department of Zoology, Islamia College Peshawar Khyber Pakhtunkwa Pakistan.
Trop Biomed. 2020 Dec 1;37(4):1000-1007. doi: 10.47665/tb.37.4.1000.
Hemophilia is a rare bleeding disorder that needs plasma or clotting factor concentrate transfusion. Therefore chances of blood-borne pathogens like HCV transmission increase due to high prevalence in healthy donors. This study was aimed to determine the prevalence of HCV genotypes and associated risk factors in hemophilia patients of Khyber Pakhtunkhwa, Pakistan. Blood samples and data were collected from 672 hemophiliacs after proper consent obtained from each patient. Samples were analyzed for anti-HCV, HCV RNA and HCV genotype/s detection. Of the total, 22.32% (150) were anti-HCV positive, of which HCV RNA was detected in 18.45% (124) individuals. HCV genotype 3a was found with significantly higher prevalence (p<0.05) (19.35%) as compared to 2a (16.13%) and 1a (12.90%). HCV-3b and HCV-4 were found each in 3.22% samples. Dual infection of genotypes was found in 22.58% of individuals and 22.58% HCV RNA positive sampels were not typed. A total of 572 (85.12%) subjects had hemophilia A and 100 (14.88%) had hemophilia B. In hemophiliacs A the most dominant genotype was 3a (19.27%) while in hemophilia B, genotype 1a was prevalent (26.67%). Whole blood and plasma transfusion were observed as the main risk factors of HCV. It is concluded that HCV genotype 3a and 2a are prevalent in hemophilia patients of Khyber Pakhtunkhwa Pakistan and the main risk factor observed was an unscreened whole blood transfusion.
血友病是一种罕见的出血性疾病，需要输注血浆或凝血因子浓缩物。因此，由于健康供体中 HCV 的高流行率，血液传播病原体（如 HCV）传播的机会增加。本研究旨在确定巴基斯坦开伯尔-普赫图赫瓦省血友病患者中 HCV 基因型的流行情况和相关危险因素。在获得每位患者的适当同意后，从 672 名血友病患者中采集血液样本和数据。对样本进行抗 HCV、HCV RNA 和 HCV 基因型/亚型检测。在总共 672 名患者中，22.32%（150 名）抗 HCV 阳性，其中 18.45%（124 名）患者的 HCV RNA 检测呈阳性。发现 HCV 基因型 3a 的流行率明显更高（p<0.05）（19.35%），而 2a（16.13%）和 1a（12.90%）的流行率较低。在 3.22%的样本中发现 HCV-3b 和 HCV-4 各一例。22.58%的个体存在两种基因型的双重感染，22.58%的 HCV RNA 阳性样本未进行分型。共有 572 名（85.12%）患者患有血友病 A，100 名（14.88%）患者患有血友病 B。在血友病 A 患者中，最主要的基因型是 3a（19.27%），而在血友病 B 患者中，1a 基因型较为常见（26.67%）。全血和血浆输血被观察为 HCV 的主要危险因素。结论是，在巴基斯坦开伯尔-普赫图赫瓦省的血友病患者中，HCV 基因型 3a 和 2a 较为流行，观察到的主要危险因素是未经筛查的全血输血。
