Talukder Zahirul I, Underwood William, Misar Christopher G, Seiler Gerald J, Liu Yuan, Li Xuehui, Cai Xiwen, Qi Lili
Department of Plant Sciences, North Dakota State University, Fargo, ND, United States.
United States Department of Agriculture - Agricultural Research Service, Edward T. Schafer Agricultural Research Center, Fargo, ND, United States.
Front Plant Sci. 2021 Feb 3;11:617920. doi: 10.3389/fpls.2020.617920. eCollection 2020.
Basal stalk rot (BSR), caused by the fungus , is a serious disease of sunflower ( L.) in the humid temperate growing areas of the world. BSR resistance is quantitative and conditioned by multiple genes. Our objective was to dissect the BSR resistance introduced from the wild annual species using a quantitative trait loci (QTL) mapping approach. An advanced backcross population (AB-QTL) with 134 lines derived from the cross of HA 89 with a Torr. and Gray accession, PI 494573, was evaluated for BSR resistance in three field and one greenhouse growing seasons of 2017-2019. Highly significant genetic variations ( < 0.001) were observed for BSR disease incidence (DI) in all field screening tests and disease rating and area under the disease progress curve in the greenhouse. The AB-QTL population and its parental lines were genotyped using the genotyping-by-sequencing method. A genetic linkage map spanning 2,045.14 cM was constructed using 3,110 SNP markers mapped on 17 sunflower chromosomes. A total of 21 QTL associated with BSR resistance were detected on 11 chromosomes, each explaining a phenotypic variation ranging from 4.5 to 22.6%. Of the 21 QTL, eight were detected for BSR DI measured in the field, seven were detected for traits measured in the greenhouse, and six were detected from both field and greenhouse tests. Thirteen of the 21 QTL had favorable alleles from the parent conferring increased BSR resistance.
由真菌引起的基部茎腐病（BSR）是世界上湿润温带种植区向日葵（Helianthus annuus L.）的一种严重病害。BSR抗性是数量性状，由多个基因控制。我们的目标是使用数量性状位点（QTL）定位方法剖析从野生一年生物种引入的BSR抗性。对一个由HA 89与Torr.和Gray的种质PI 494573杂交产生的包含134个株系的高代回交群体（AB-QTL），在2017 - 2019年的三个田间生长季节和一个温室生长季节中进行了BSR抗性评估。在所有田间筛选试验中，观察到BSR发病率（DI）以及在温室中的病情评级和病害进展曲线下面积存在极显著的遗传变异（P < 0.001）。使用简化基因组测序方法对AB-QTL群体及其亲本系进行基因分型。利用定位在17条向日葵染色体上的3110个单核苷酸多态性（SNP）标记构建了一个跨度为2045.14厘摩（cM）的遗传连锁图谱。在11条染色体上共检测到21个与BSR抗性相关的QTL，每个QTL解释的表型变异范围为4.5%至22.6%。在这21个QTL中，8个是针对田间测量的BSR DI检测到的，7个是针对温室中测量的性状检测到的，6个是从田间和温室试验中都检测到的。21个QTL中的13个具有来自PI 494573亲本的有利等位基因，赋予了增强的BSR抗性。
