Wang Jialin, Liu Zhendong, Zhang Cheng, Wang Hongbo, Li Ang, Liu Binfeng, Lian Xiaoyu, Ren Zhishuai, Zhang Wang, Wang Yanbiao, Zhang Bo, Pang Bo, Gao Yanzheng
Zhengzhou University People's Hospital, Henan Provincial People's Hospital, Zhengzhou, Henan, China.
Department of Microbiome Laboratory, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, Henan, China.
PeerJ. 2021 Feb 8;9:e10820. doi: 10.7717/peerj.10820. eCollection 2021.
Homeobox D11 () plays an important role in a variety of cancers, but its precise role in gliomas remains unclear. This study aimed to explore the relationship between and gliomas by combining bioinformatics methods with basic experimental validation.
Obtain gene expression information and clinical information of glioma and non-tumor brain tissue samples from multiple public databases such as TCGA (666 glioma samples), CGGA (749 glioma samples), GEPIA(163 glioblastoma samples and 207 normal control samples), GEO (GSE4290 and GSE15824). Nine cases of glioma tissue and five cases of normal control brain tissue were collected from the clinical department of Henan Provincial People's Hospital for further verification. A series of bioinformatic analysis methods were used to confirm the relationship between expression and overall survival and clinical molecular characteristics of patients with glioma. RT-qPCR was used to verify the change of expression level of in glioma cells and tissues. MTT assay, colony formation assay, wound-healing assay, immunofluorescence staining, flow cytometry and western blotting were used to detect the effect of on the biological behavior of glioma cell line U251.
The high expression of was significantly related to age, World Health Organization (WHO) grade, chemotherapy status, histological type, and even 1p19q codeletion data and isocitrate dehydrogenase (IDH) mutation. , as an independent risk factor, reduces the overall survival of glioma patients and has diagnostic value for the prognosis of glioma. Gene Set Enrichment Analysis (GSEA) showed that was significantly enriched in cell signaling pathway such as cell cycle, DNA replication and so on. Finally, we confirmed that the knockout of can inhibit the proliferation and invasion of U251 glioma cells, and change the biological behavior of tumor cells by preventing the progression of cell cycle.
may be used as a candidate biomarker for the clinical application of targeted drug and prognostic assessment treatment of glioma. In addition, This study will help to explore the pathological mechanism of glioma.
同源盒D11（）在多种癌症中发挥重要作用，但其在胶质瘤中的具体作用仍不清楚。本研究旨在通过生物信息学方法与基础实验验证相结合，探讨与胶质瘤的关系。
从多个公共数据库获取胶质瘤和非肿瘤脑组织样本的基因表达信息及临床信息，如TCGA（666例胶质瘤样本）、CGGA（749例胶质瘤样本）、GEPIA（163例胶质母细胞瘤样本和207例正常对照样本）、GEO（GSE-4290和GSE-15824）。从河南省人民医院临床科室收集9例胶质瘤组织和5例正常对照脑组织进行进一步验证。采用一系列生物信息学分析方法，证实表达与胶质瘤患者总生存期及临床分子特征之间的关系。采用RT-qPCR验证胶质瘤细胞和组织中表达水平的变化。采用MTT法、集落形成法、伤口愈合法、免疫荧光染色、流式细胞术和蛋白质印迹法检测对胶质瘤细胞系U251生物学行为的影响。
的高表达与年龄、世界卫生组织（WHO）分级、化疗状态组织学类型，甚至1p19q共缺失数据和异柠檬酸脱氢酶（IDH）突变显著相关。作为独立危险因素，降低了胶质瘤患者的总生存期，对胶质瘤预后具有诊断价值。基因集富集分析（GSEA）显示，在细胞周期、DNA复制等细胞信号通路中显著富集。最后，我们证实敲除可抑制U251胶质瘤细胞的增殖和侵袭，并通过阻止细胞周期进程改变肿瘤细胞的生物学行为。
可作为胶质瘤靶向药物临床应用和预后评估治疗的候选生物标志物。此外，本研究将有助于探索胶质瘤的病理机制。
Zotero 插件
