Mohd Amnan Muhammad Asyraf, Pua Teen-Lee, Lau Su-Ee, Tan Boon Chin, Yamaguchi Hisateru, Hitachi Keisuke, Tsuchida Kunihiro, Komatsu Setsuko
Centre for Research in Biotechnology for Agriculture, University of Malaya, Kuala Lumpur, Malaysia.
Department of Medical Technology, Yokkaichi Nursing and Medical Care University, Yokkaichi, Japan.
PeerJ. 2021 Feb 9;9:e10879. doi: 10.7717/peerj.10879. eCollection 2021.
Drought is one of the severe environmental stresses threatening agriculture around the globe. Nitric oxide plays diverse roles in plant growth and defensive responses. Despite a few studies supporting the role of nitric oxide in plants under drought responses, little is known about its pivotal molecular amendment in the regulation of stress signaling. In this study, a label-free nano-liquid chromatography-mass spectrometry approach was used to determine the effects of sodium nitroprusside (SNP) on polyethylene glycol (PEG)-induced osmotic stress in banana roots. Plant treatment with SNP improved plant growth and reduced the percentage of yellow leaves. A total of 30 and 90 proteins were differentially identified in PEG+SNP against PEG and PEG+SNP against the control, respectively. The majority of proteins differing between them were related to carbohydrate and energy metabolisms. Antioxidant enzyme activities, such as superoxide dismutase and ascorbate peroxidase, decreased in SNP-treated banana roots compared to PEG-treated banana. These results suggest that the nitric oxide-induced osmotic stress tolerance could be associated with improved carbohydrate and energy metabolism capability in higher plants.
干旱是威胁全球农业的严重环境胁迫之一。一氧化氮在植物生长和防御反应中发挥着多种作用。尽管有一些研究支持一氧化氮在植物干旱反应中的作用，但对于其在应激信号调控中的关键分子修饰知之甚少。在本研究中，采用无标记纳米液相色谱-质谱联用方法来确定硝普钠（SNP）对聚乙二醇（PEG）诱导的香蕉根渗透胁迫的影响。用SNP处理植物可促进植物生长并降低黄叶百分比。分别在PEG+SNP与PEG以及PEG+SNP与对照之间差异鉴定出30种和90种蛋白质。它们之间大多数差异蛋白质与碳水化合物和能量代谢有关。与PEG处理的香蕉相比，SNP处理的香蕉根中超氧化物歧化酶和抗坏血酸过氧化物酶等抗氧化酶活性降低。这些结果表明，一氧化氮诱导的渗透胁迫耐受性可能与高等植物中碳水化合物和能量代谢能力的提高有关。
