Fu Tingting, Knittelfelder Oskar, Geffard Olivier, Clément Yohann, Testet Eric, Elie Nicolas, Touboul David, Abbaci Khedidja, Shevchenko Andrej, Lemoine Jerome, Chaumot Arnaud, Salvador Arnaud, Degli-Esposti Davide, Ayciriex Sophie
Univ Lyon, CNRS, Université Claude Bernard Lyon 1, Institut des Sciences Analytiques, UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, France.
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307 Dresden, Germany.
iScience. 2021 Jan 29;24(2):102115. doi: 10.1016/j.isci.2021.102115. eCollection 2021 Feb 19.
Sentinel species are playing an indispensable role in monitoring environmental pollution in aquatic ecosystems. Many pollutants found in water prove to be endocrine disrupting chemicals that could cause disruptions in lipid homeostasis in aquatic species. A comprehensive profiling of the lipidome of these species is thus an essential step toward understanding the mechanism of toxicity induced by pollutants. Both the composition and spatial distribution of lipids in freshwater crustacean were extensively examined herein. The baseline lipidome of gammarids of different sex and reproductive stages was established by high throughput shotgun lipidomics. Spatial lipid mapping by high resolution mass spectrometry imaging led to the discovery of sulfate-based lipids in hepatopancreas and their accumulation in mature oocytes. A diverse and dynamic lipid composition in was uncovered, which deepens our understanding of the biochemical changes during development and which could serve as a reference for future ecotoxicological studies.
指示物种在监测水生生态系统中的环境污染方面发挥着不可或缺的作用。水中发现的许多污染物被证明是内分泌干扰化学物质，可能导致水生物种的脂质稳态紊乱。因此，全面分析这些物种的脂质组是了解污染物诱导毒性机制的重要一步。本文广泛研究了淡水甲壳类动物脂质的组成和空间分布。通过高通量鸟枪法脂质组学建立了不同性别和生殖阶段的钩虾的基线脂质组。通过高分辨率质谱成像进行的空间脂质图谱分析发现了肝胰腺中基于硫酸盐的脂质及其在成熟卵母细胞中的积累。揭示了丰富多样且动态变化的脂质组成，这加深了我们对发育过程中生化变化的理解，并可为未来的生态毒理学研究提供参考。
