增强鸟枪法脂质组学以全面分析细胞脂质组的新策略。
Novel strategies for enhancing shotgun lipidomics for comprehensive analysis of cellular lipidomes.
作者信息
Hu Changfeng, Wang Chunyan, He Lijiao, Han Xianlin
机构信息
College of Basic Medical Sciences, Zhejiang Chinese Medical University, 548 Bingwen Road, Hangzhou, Zhejiang 310053, China.
Barshop Institute for Longevity and Aging Research, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229, USA.
出版信息
Trends Analyt Chem. 2019 Nov;120. doi: 10.1016/j.trac.2018.11.028. Epub 2018 Nov 27.
Abstract
Shotgun lipidomics is one of the most powerful tools in analysis of cellular lipidomes in lipidomics, which directly analyzes lipids from lipid extracts of diverse biological samples with high accuracy/precision. However, despite its great advances in high throughput analysis of cellular lipidomes, low coverage of poorly ionized lipids, especially those species in very low abundance, and some types of isomers within complex lipid extracts by shotgun lipidomics remains a huge challenge. In the past few years, many strategies have been developed to enhance shotgun lipidomics for comprehensive analysis of lipid species. Chemical derivatization represents one of the most attractive and effective strategies, already receiving considerable attention. This review focuses on novel advanced derivatization strategies for enhancing shotgun lipidomics. It is anticipated that with the development of enhanced strategies, shotgun lipidomics can make greater contributions to biological and biomedical research.
摘要
鸟枪法脂质组学是脂质组学中分析细胞脂质组的最强大工具之一，它能以高精度/高准确度直接分析来自各种生物样品脂质提取物中的脂质。然而，尽管鸟枪法脂质组学在细胞脂质组的高通量分析方面取得了巨大进展，但对于电离性差的脂质，尤其是那些丰度极低的脂质种类，以及复杂脂质提取物中的某些异构体类型，其覆盖率较低，这仍然是一个巨大的挑战。在过去几年中，已经开发了许多策略来增强鸟枪法脂质组学以全面分析脂质种类。化学衍生化是最具吸引力和最有效的策略之一，已经受到了相当多的关注。本综述重点关注增强鸟枪法脂质组学的新型先进衍生化策略。预计随着增强策略的发展，鸟枪法脂质组学能够为生物学和生物医学研究做出更大贡献。
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