Dwia Pertiwi Yunialthy, Chikama Taiichiro, Sueoka Kentaro, Ko Ji-Ae, Kiuchi Yoshiaki, Onodera Makoto, Sakaguchi Takemasa
Department of Ophthalmology and Visual Science, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, 734-8551, Japan.
Faculty of Medicine, Hasanuddin University, Makassar, South Sulawesi, 90245, Indonesia.
Lasers Surg Med. 2021 Jul;53(5):695-702. doi: 10.1002/lsm.23355. Epub 2020 Dec 8.
Acanthamoeba keratitis is a sight-threatening infectious disease that is difficult to treat. The aim of this study was to evaluate TONS504 (cationic chlorin derivative photosensitizer)-mediated photodynamic antimicrobial chemotherapy (PACT) in vivo as a potential treatment for Acanthamoeba keratitis.
STUDY DESIGN/MATERIALS AND METHODS: Acanthamoeba keratitis was induced by soft contact lenses incubated with 1 × 10 /ml Acanthamoeba castellanii, which were placed over debrided corneas with temporary tarsorrhaphy. Thirty-eight male Japanese white rabbits were randomly divided into three groups (normal eye, no treatment, and treatment groups). TONS504 was administered as eye drops at 1 mg/ml, followed by light-emitting diode irradiation after the establishment of keratitis at 7 days after infectious contact lens exposure. All animals were evaluated under a slit-lamp microscope every 3 days for 6 days after the treatment. Clinical scores based on corneal epithelial defects detected by fluorescein staining, stromal opacity edema, and vascular infiltration into the cornea were determined. After 6 days, the eyes were enucleated for histopathological analysis.
Clinical signs of infection in the treatment group were markedly reduced for up to 6 days after treatment. Histopathology showed a regular arrangement of stromal fibers and a small number of inflammatory cells in 58% of the corneas. However, 42% of corneas in the treatment group showed infiltrating neutrophils and irregular alignment of stromal collagen fibers.
Our TONS504-PACT achieved complete recovery from keratitis in 58% of the rabbit models. Further studies are required to determine the conditions for the maximal effectiveness of our TONS504-PACT for Acanthamoeba keratitis. Lasers Surg. Med. © 2020 Wiley Periodicals LLC.
棘阿米巴角膜炎是一种威胁视力的难治性感染性疾病。本研究旨在评估TONS504（阳离子氯衍生物光敏剂）介导的光动力抗菌化疗（PACT）在体内作为棘阿米巴角膜炎潜在治疗方法的效果。
研究设计/材料与方法：用与1×10⁶/ml卡氏棘阿米巴孵育的软性隐形眼镜诱发棘阿米巴角膜炎，将其置于清创后的角膜上并临时睑裂缝合。38只雄性日本白兔随机分为三组（正常眼组、未治疗组和治疗组）。以1mg/ml的滴眼液形式给予TONS504，在感染性隐形眼镜暴露7天后角膜炎形成后进行发光二极管照射。治疗后6天内每3天在裂隙灯显微镜下对所有动物进行评估。根据荧光素染色检测到的角膜上皮缺损、基质混浊水肿以及角膜血管浸润确定临床评分。6天后摘除眼球进行组织病理学分析。
治疗组治疗后长达6天感染的临床症状明显减轻。组织病理学显示，58%的角膜基质纤维排列规则，炎症细胞数量少。然而，治疗组42%的角膜显示有中性粒细胞浸润和基质胶原纤维排列不规则。
我们的TONS504-PACT使58%的兔模型角膜炎完全恢复。需要进一步研究以确定我们的TONS504-PACT治疗棘阿米巴角膜炎的最大有效性条件。激光外科医学。©2020威利期刊有限责任公司。
