Departments of Ophthalmology, Gazi University , Ankara, Turkey.
Departments of Medical Microbiology, School of Medicine, Gazi University , Ankara, Turkey.
Curr Eye Res. 2020 Oct;45(10):1205-1210. doi: 10.1080/02713683.2020.1731830. Epub 2020 Feb 27.
: To evaluate the in vivo efficacy of rose bengal (RB)-mediated photodynamic antimicrobial therapy (PDAT) for treatment of keratitis (AK). : An animal (rabbit) AK model was successfully achieved via intrastromal inoculation of a suspension of cells and trophozoites. Prior to RB-PDAT (pre-treatment, day-5), the severity of the induced corneal infection was graded numerically for epithelial defects, stromal edema, neovascularity, and stromal opacity/infiltration. The right eyes of rabbits (n = 18) were divided equally into three groups (n = 6/group): control (no treatment); 0.1% RB+518 nm irradiation (5.4 J/cm); and 0.2% RB+518 nm irradiation (5.4 J/cm). On post-treatment day-5, animals were euthanized, after which corneal buttons were excised and submitted for real-time polymerase chain reaction (RT-PCR) analysis. : Post-treatment clinical scores of the 0.1 and 0.2% RB groups indicated significant improvement compared to control group scores (pre-treatment clinical scores; 5.17 ± 0.98, 7.50 ± 0.62, and 6.17 ± 0.70 and post-treatment clinical scores; 4.50 ± 0.56, ( = .043), 3.50 ± 0.99 ( = .039), 6.83 ± 1.66 ( = .34), respectively). RT-PCR analysis revealed that the mean cycle threshold (Ct) values were significantly higher in treated-group corneas compared to control-group corneas, with no significant differences between treated-groups (Mean Ct values; 34.33, 34.5, and 29.67 for 0.1 and 0.2% RB, and control groups). There was a statistically significant negative correlation between post-treatment clinical scores and Ct values ( = -0.474, -value 0.047). : Our results demonstrate that RB-PDAT is effective in decreasing the parasitic load and clinical severity of AK.
: 为了评估孟加拉玫瑰红（RB）介导的光动力抗菌疗法（PDAT）治疗角膜炎（AK）的体内疗效。: 通过角膜基质内接种细胞和滋养体悬浮液成功建立了动物（兔）AK 模型。在 RB-PDAT（预处理，第 5 天）之前，通过数字评分评估诱导的角膜感染的严重程度，包括上皮缺损、基质水肿、新生血管形成和基质混浊/浸润。将兔子的右眼（n=18）平均分为三组（n=6/组）：对照组（无治疗）；0.1%RB+518nm 照射（5.4J/cm）；和 0.2%RB+518nm 照射（5.4J/cm）。在第 5 天的治疗后，处死动物，然后切除角膜瓣并进行实时聚合酶链反应（RT-PCR）分析。: 与对照组相比，0.1%和 0.2%RB 组治疗后的临床评分显著改善（预处理临床评分分别为 5.17±0.98、7.50±0.62 和 6.17±0.70，治疗后临床评分分别为 4.50±0.56、0.043）、3.50±0.99（ =0.039）、6.83±1.66（ =0.34））。RT-PCR 分析显示，与对照组相比，治疗组角膜的平均循环阈值（Ct）值显著升高，治疗组之间无显著差异（0.1%和 0.2%RB 组和对照组的平均 Ct 值分别为 34.33、34.5 和 29.67）。治疗后临床评分与 Ct 值之间呈显著负相关（ =-0.474，-值 0.047）。: 我们的结果表明，RB-PDAT 可有效降低 AK 的寄生虫负荷和临床严重程度。
