从人和肉鸡中分离出的[具体细菌名称未给出]的喹诺酮耐药性和抗菌药物耐药性谱的分子特征分析
Molecular characterization of quinolone resistance and antimicrobial resistance profiles of and isolated from human and broiler chickens.
作者信息
Haeili Mehri, Salehzeinali Hila, Mirzaei Somayyeh, Pishnian Zeinab, Ahmadi Amin
机构信息
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran.
出版信息
Int J Environ Health Res. 2022 Jun;32(6):1382-1392. doi: 10.1080/09603123.2021.1885632. Epub 2021 Feb 21.
This study characterized quinolone (Q) resistance determinants in a series of ( = 26) and ( = 19) isolates of human and animal origin. The presence of plasmid-mediated quinolone resistance (PMQR) and carabpenemase genes was examined by PCR. The quinolone resistance-determining regions (QRDRs) of and genes were sequenced. Thirty-three isolates had ciprofloxacin MIC≥8 mg/l. About 34.6% and 10.5% of and isolates were ESBL producers respectively. The PMQR genes were detected in 77% ( = 35) of isolates. The was the most prevalent PMQR gene being identified in all isolates, followed by (34.6%), (23%) and (7.7%). The most frequently detected gene among isolates was (36.8%) followed by (10.5%) and (5.2%). All Q resistant isolates harbored amino acid substitutions in both GyrA and ParC QRDRs. High prevalence of PMQR genes among food-producing animal isolates is an issue of great concern.
本研究对一系列26株人源和19株动物源的大肠埃希菌和肺炎克雷伯菌分离株中的喹诺酮（Q）耐药决定因素进行了表征。通过PCR检测质粒介导的喹诺酮耐药性（PMQR）和碳青霉烯酶基因的存在情况。对大肠埃希菌和肺炎克雷伯菌基因的喹诺酮耐药决定区（QRDRs）进行了测序。33株分离株的环丙沙星MIC≥8 mg/l。大肠埃希菌和肺炎克雷伯菌分离株中分别有34.6%和10.5%为超广谱β-内酰胺酶（ESBL）产生菌。77%（n = 35）的分离株检测到PMQR基因。qnrS是所有分离株中最常见的PMQR基因，其次是qnrB（34.6%）、qepA（23%）和aac(6′)-Ib-cr（7.7%）。肺炎克雷伯菌分离株中最常检测到的基因是qnrB（36.8%），其次是qnrS（10.5%）和qepA（5.2%）。所有对Q耐药的分离株在GyrA和ParC QRDRs中均存在氨基酸替换。食品生产动物分离株中PMQR基因的高流行率是一个令人高度关注的问题。
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