在原代白血病细胞中，TNF-α促进了TGF-β1对Gli2表达的抑制作用。
Inhibition of TGF-β1 on Gli2 expression was promoted by TNF-α in primary leukemia cells.
作者信息
Li Zhe, Mao Shudan, Zhang Ning
机构信息
Department of Hematology, The First Affiliated Hospital of Jinzhou Medical University, Liaoning, P. R. China.
出版信息
J Recept Signal Transduct Res. 2022 Apr;42(2):169-172. doi: 10.1080/10799893.2021.1881555. Epub 2021 Feb 22.
PURPOSE
Hedgehog (Hh) signaling pathway regulates a variety of tumors-related diseases including leukemia. Whether inhibition of TGF-β1 on Gli2 expression is promoted by TNF-α in primary leukemia cells remains to be determined.
METHODS
Primary leukemia cells were treated with TGF-β1, TNF-α or SIS3 at different concentrations. Gli2 expression was detected by quantitative real-time PCR and western blot analyses.
RESULTS
We found that TGF-β significantly decreased Gli2 expression, and co-treatment with TNF-αfurther decreased Gli2 expression in primary leukemia cells. TNF-α can increased TGF-βRI and TGF-βRII protein expression in primary leukemia cells, while SIS3 inhibited the effect of TGF-β.
CONCLUSION
Our results suggest that Gli2 expression in primary leukemia cells is induced by TGF-β in a Smad3-dependent manner, and independent of Hh receptor signaling.
目的
刺猬信号通路（Hh）调控包括白血病在内的多种肿瘤相关疾病。肿瘤坏死因子-α（TNF-α）是否促进原发性白血病细胞中转化生长因子-β1（TGF-β1）对Gli2表达的抑制作用仍有待确定。
方法
用不同浓度的TGF-β1、TNF-α或SIS3处理原发性白血病细胞。通过定量实时聚合酶链反应和蛋白质免疫印迹分析检测Gli2表达。
结果
我们发现TGF-β显著降低原发性白血病细胞中Gli2的表达，并且在原发性白血病细胞中，TNF-α与TGF-β1共同处理可进一步降低Gli2的表达。TNF-α可增加原发性白血病细胞中TGF-βRI和TGF-βRII蛋白的表达，而SIS3可抑制TGF-β的作用。
结论
我们的结果表明，原发性白血病细胞中Gli2的表达由TGF-β以Smad3依赖的方式诱导，且独立于Hh受体信号传导。
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