Khor G L, Alexander J C, Lumsden J H, Losos G J
Can J Comp Med. 1977 Oct;41(4):428-34.
A safety evaluation of Aspergillus fumigatus I21, grown in a cassava carbohydrate and salts medium, was undertaken. Male weanling rats were fed the fungus at 20, 30 and 40% of the diet for 90 days. A control group was given soybean oil meal as the sole source of protein. Weekly determinations of the body weights and feed consumptions were made. A few days prior to termination of the feeding study, a kidney function test was undertaken on the rats. At the end of the feeding period hematology, blood biochemistry, urine analyses and histopathology studies of various tissues were carried out, and organs were weighed. Rats fed A. fumigatus I21 gained less weight than the controls, but kidney weights were increased. Increases in serum alkaline phosphatase and glutamic-oxaloacetic transaminase were not related to dose level. The blood urea nitrogen was increased for the rats fed 40% of the fungus. Rats fed 30 and 40% of the fungus I21 showed a significant drop in albumin. Deficiency in methionine or other essential amino acids through a limited feed consumption may have caused a decrease in albumin synthesis. Rats fed the highest level of the fungus showed increases in neutrophils and monocytes concomitant with decreases in lymphocytes and eosinophils which may be a response to stress. The urine analyses did not reveal any significant differences. The test rats were capable of concentrating urine adequately when deprived of water for 24 hours. No significant differences between the control and experimental groups were found by histopathological examinations.
对在木薯碳水化合物和盐类培养基中生长的烟曲霉I21进行了安全性评估。将雄性断乳大鼠分别饲喂含有20%、30%和40%该真菌的饲料，持续90天。对照组给予豆粕作为唯一的蛋白质来源。每周测定大鼠体重和饲料消耗量。在喂养研究结束前几天，对大鼠进行了肾功能测试。喂养期结束时，对大鼠进行了血液学、血液生化、尿液分析以及各种组织的组织病理学研究，并对器官进行称重。饲喂烟曲霉I21的大鼠体重增长低于对照组，但肾脏重量增加。血清碱性磷酸酶和谷草转氨酶的升高与剂量水平无关。饲喂40%真菌的大鼠血尿素氮升高。饲喂30%和40%真菌I21的大鼠白蛋白显著下降。由于饲料摄入量有限导致蛋氨酸或其他必需氨基酸缺乏，可能导致白蛋白合成减少。饲喂最高剂量真菌的大鼠中性粒细胞和单核细胞增加，同时淋巴细胞和嗜酸性粒细胞减少，这可能是对压力的一种反应。尿液分析未发现任何显著差异。当剥夺试验大鼠24小时饮水时，它们能够充分浓缩尿液。组织病理学检查未发现对照组和实验组之间有显著差异。
