外泌体miR-23a和miR-192，2型糖尿病的潜在诊断生物标志物。
Exosomal miR-23a and miR-192, Potential Diagnostic Biomarkers for Type 2 Diabetes.
作者信息
Liu Chunxing, Gao Yonghui, Wu Jianfen, Zou Jian
出版信息
Clin Lab. 2021 Feb 1;67(2). doi: 10.7754/Clin.Lab.2020.200612.
BACKGROUND
Type 2 diabetes mellitus (T2D) is a common chronic disease, which lacks a specific diagnostic method. A substantial body of literature has demonstrated that the molecular constituents of extracellular vesicles (EVs) are promising as a novel biomarker for clinical diagnosis, especially exosomal miRNAs in biological fluids.
METHODS
To find a diagnostic biomarker for T2D, we isolated exosomes from plasma and then quantitative PCR (qPCR) was used to detect the miRNA expression in plasma and exosomes from control subjects and T2D subjects. The ROC (receiver operating characteristic) curve and AUC (area under curve) were used to evaluate the diagnostic value of exosomal miRNAs.
RESULTS
We found the exosomal levels of miR-23a and miR-192 were both significantly higher in T2D subjects. The AUC of miR-23a and miR-192 were 0.828 and 0.717, respectively. Further bioinformatics analysis was performed to speculate possible mechanisms.
CONCLUSIONS
In conclusion, exosomal miR-23a and miR-192 have good potential as diagnostic biomarkers for type 2 diabetes.
背景
2型糖尿病（T2D）是一种常见的慢性病，缺乏特异性诊断方法。大量文献表明，细胞外囊泡（EVs）的分子成分有望成为临床诊断的新型生物标志物，尤其是生物体液中的外泌体微小RNA（miRNAs）。
方法
为寻找T2D的诊断生物标志物，我们从血浆中分离出外泌体，然后采用定量聚合酶链反应（qPCR）检测对照受试者和T2D受试者血浆及外泌体中的miRNA表达。采用受试者工作特征（ROC）曲线和曲线下面积（AUC）评估外泌体miRNAs的诊断价值。
结果
我们发现T2D受试者中miR-23a和miR-192的外泌体水平均显著升高。miR-23a和miR-192的AUC分别为0.828和0.717。进行了进一步的生物信息学分析以推测可能的机制。
结论
总之，外泌体miR-23a和miR-192作为2型糖尿病的诊断生物标志物具有良好的潜力。
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