Department of Biology, University of Puerto Rico, San Juan, Puerto Rico.
Institute of Neurobiology, University of Puerto Rico, San Juan, Puerto Rico.
PLoS One. 2021 Feb 22;16(2):e0247382. doi: 10.1371/journal.pone.0247382. eCollection 2021.
The species Chironomus sp. "Florida" has several qualities that make it a potential aquatic laboratory model to be used in Puerto Rico. Its use as such, however, requires a rearing protocol and life cycle description not previously reported. The present study addresses this lack of information by first describing a rearing method obtained through three years of observations. Next we describe and discuss the life cycle and the effects of temperature and feeding on development. The species has a short life cycle (typically 11 days) and larval stages easily identified using body measurements. Temperature affects the duration of the life cycle, with warm temperatures producing faster development than cold temperatures. The effects of different food concentrations vary: in large water volumes, concentrations of 2 mg/larva/day produce faster developmental times, but at low water volumes, small food concentrations of 0.5 mg/larva/day produce faster developmental times. The rearing protocol and life cycle parameters presented in this study are intended to promote the use of this species as a laboratory model. The fast development of Chironomus sp. "Florida" makes it ideal for toxicological studies.
“佛罗里达”摇蚊（Chironomus sp. "Florida"）具有多种特性，使其成为波多黎各潜在的水生实验室模型。然而，要将其用作模型，需要制定以前未曾报告过的饲养方案和生命周期描述。本研究通过三年的观察，首先描述了一种饲养方法，以填补这方面的信息空白。接下来，我们描述并讨论了生命周期以及温度和饲养对发育的影响。该物种的生命周期较短（通常为 11 天），幼虫阶段可以通过身体测量轻松识别。温度会影响生命周期的持续时间，高温比低温产生更快的发育。不同食物浓度的影响不同：在大水体中，每天每幼虫 2 毫克的浓度会产生更快的发育时间，但在小水体中，每天每幼虫 0.5 毫克的低浓度食物会产生更快的发育时间。本研究中提出的饲养方案和生命周期参数旨在促进该物种作为实验室模型的使用。“佛罗里达”摇蚊的快速发育使其成为毒理学研究的理想选择。
