Unidad de Citología e Histología. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia. Facultad de Biociencias, Institut de Neurociències. Universidad Autónoma de Barcelona, Bellaterra, Barcelona, Spain.
Anat Rec (Hoboken). 2021 Dec;304(12):2856-2864. doi: 10.1002/ar.24607. Epub 2021 Mar 1.
The aim of this report is to determine whether the times of neuron origin and neurogenetic gradients of PCs and Deep cerebellar nucli (DCN) glutamatergic neurons are different between mice and rats. Purkinje cells (PCs) were analyzed in each compartment of the cerebellar cortex (vermis, paravermis, medial, and lateral hemispheres), and deep glutamatergic neurons at the level of the medialis, interpositus, and lateralis nuclei. Tritiated thymidine ([ H]TdR) autoradiography was applied on sections. The experimental rodents were the offspring of pregnant dams injected with [ H]TdR on embryonic days (E) 11-12, E12-13, E13-14, E14-15, E15-16, and E16-17. Our results indicate that systematic differences exist in the pattern of neurogenesis and the spatial location of cerebellar PCs and deep glutamatergic neurons between mice and rats. In mice, PCs and deep glutamatergic neurons neurogenesis extend from E10 to E14, with a predominance of neurogenesis on E12 for PCs, and on E12, E11, and E10 for the medialis, interpositus, and lateralis neurons, respectively. When neurogenesis in rats was considered, the data reveal that PCs and deep glutamatergic neurons production extends from E12 to E16, with a peak of production on E14 for PCs, and on E14, E13, and E12 for the medialis, interpositus, and lateralis neurons, respectively. Current data also indicate that, both in mice and rats, both types of macroneurons are generated according to a lateral-to-medial gradient. Thus, the lateral hemisphere and the lateralis nucleus present more early-generated neurons than the vermis and the medialis nucleus, which in their turn have more late-produced neurons.
本报告旨在确定在小鼠和大鼠中，浦肯野细胞 (PCs) 和深部小脑核 (DCN) 谷氨酸能神经元的起源时间和神经发生梯度是否不同。对小脑皮质的每个隔室 (蚓部、副蚓部、内侧和外侧半球) 中的 PCs 和深部谷氨酸能神经元在中间核、间位核和外侧核水平进行了分析。使用氚标记胸腺嘧啶核苷 ([H]TdR) 放射自显影术对切片进行分析。实验啮齿动物是在胚胎第 11-12、12-13、13-14、14-15、15-16 和 16-17 天注射 [H]TdR 的怀孕母鼠的后代。我们的结果表明，在小鼠和大鼠中，神经发生的模式和小脑 PCs 和深部谷氨酸能神经元的空间位置存在系统差异。在小鼠中，PCs 和深部谷氨酸能神经元的神经发生从 E10 延伸到 E14，以 E12 时的神经发生为主，而对于中间核、间位核和外侧核神经元，分别以 E12、E11 和 E10 时的神经发生为主。当考虑大鼠的神经发生时，数据显示 PCs 和深部谷氨酸能神经元的产生从 E12 延伸到 E16，以 E14 时的产生为主，而对于中间核、间位核和外侧核神经元，分别以 E14、E13 和 E12 时的产生为主。目前的数据还表明，无论是在小鼠还是大鼠中，这两种类型的大型神经元都是按照从外侧到内侧的梯度产生的。因此，外侧半球和外侧核比蚓部和中间核产生更多的早期神经元，而蚓部和中间核又产生更多的晚期神经元。
