Altman J, Bayer S A
J Comp Neurol. 1980 Dec 15;194(4):905-29. doi: 10.1002/cne.901940411.
Groups of pregnant rats were injected with two successive daily doses of 3H-thymidine from gestational day 12 and 13 (E12 + 13) until the day before parturition (E21 + 22) in order to label in their embryos the proliferating precursors of neurons. At 60 days of age the proportion of neurons generated (or no longer labeled) on specific embryonic days was determined quantitatively in 14 nuclei of the pontine region. Peak production time of neurons of the trigeminal mesencephalic nucleus was on day E11 or earlier, with a small proportion generated on day E12. Peak production time of the trigeminal motor neurons was on day E12, with a small proportion produced earlier. Neurons of the principal sensory nucleus were generated between days E13 and E16, with a peak on day E14; the late-produced neurons tended to belong to a class of intermediate and large cells. The bulk of the neurons of the supratrigeminal and infratrigeminal nuclei arose on days E15 and E16. Neurons of the locus coeruleus are produced mostly on day E12, with about 20% of the cells arising on day E13. The bulk of the neurons of the dorsal tegmental nucleus (Gudden's) are produced between days E13 and E15, whereas most of the neurons of the deep (ventral) tegmental nucleus are produced on day E15. A dorsal-to-caudal gradient was also obtained between the dorsal and ventral nuclei of the lateral lemniscus, the neurons of the former being generated between days E12 and E15; the latter, between days E13 and E17. The neurons of both the pars lateralis and the pars medialis of the parabrachial nucleus were produced simultaneously between days E13 and E15, with a peak on day E13. The heterogeneous collection of neurons of the pontine paramedial reticular formation was produced for day E11 (or earlier) until day E15. Finally, the neurons of the raphe pontis parvicellularis were generated at an even rate between days E13 and E15, whereas the bulk of the neurons of the raphe pontis magnocellularis were produced on days E15 and E16. On the basis of datings obtained for 9 subdivisions of the entire brain stem trigeminal complex, hypotheses were offered of the cytogenetic components of the system. The sequence of neuron production in the dorsal and deep tegmental nuclei was related to their connections with divisions of the mammillary and habenular nuclei on a "first come-first serve" basis.
将怀孕大鼠分为几组，从妊娠第12天和第13天（E12 + 13）开始，每天连续注射两次3H - 胸腺嘧啶核苷，直至分娩前一天（E21 + 22），以便标记其胚胎中神经元的增殖前体。在60日龄时，定量测定脑桥区域14个核中在特定胚胎日产生（或不再标记）的神经元比例。三叉神经中脑核神经元的产生高峰时间是在E11天或更早，E12天产生的比例较小。三叉神经运动神经元的产生高峰时间是在E12天，较早产生的比例较小。主要感觉核的神经元在E13天至E16天之间产生，高峰在E14天；后期产生的神经元倾向于属于一类中型和大型细胞。三叉上核和三叉下核的大部分神经元在E15天和E16天产生。蓝斑核的神经元大多在E12天产生，约20%的细胞在E13天产生。被盖背核（古登氏核）的大部分神经元在E13天至E15天之间产生，而深部（腹侧）被盖核的大多数神经元在E15天产生。外侧丘系背核和腹核之间也获得了从背到尾的梯度，前者的神经元在E12天至E15天之间产生；后者在E13天至E17天之间产生。臂旁核外侧部和内侧部的神经元在E13天至E15天之间同时产生，高峰在E13天。脑桥旁正中网状结构的神经元异质集合在E11天（或更早）至E15天产生。最后，脑桥缝核小细胞的神经元在E13天至E15天之间以均匀的速率产生，而脑桥缝核大细胞的大部分神经元在E15天和E16天产生。根据对整个脑干三叉神经复合体9个亚区的测定结果，提出了该系统细胞遗传学成分的假说。被盖背核和深部被盖核中神经元产生的顺序与其与乳头体核和缰核各部分的连接在“先来先服务”的基础上相关。
