采用 Ion Torrent 系统的 FusionPlex 检测到弱证据融合候选物。
Weak-evidence Fusion Candidates Detected by a FusionPlex Assay Using the Ion Torrent System.
机构信息
Department of Pathology and Molecular Diagnostics, Aichi Cancer Center, Nagoya, Japan;
Department of Respiratory Medicine, Kobe Central Hospital, Kobe, Japan.
出版信息
In Vivo. 2021 Mar-Apr;35(2):993-998. doi: 10.21873/invivo.12342.
BACKGROUND/AIM: The Archer FusionPlex platform is widely used for comprehensive fusion-gene detection in cancer tissues. This platform separately displays results for strong-evidence and weak-evidence fusion candidates (WEFCs). Distinctive fusion patterns are frequently found in the weak-evidence category and information about the patterns is clinically essential.
PATIENTS AND METHODS
We describe the type and frequency of WEFCs observed using the FusionPlex Sarcoma Panel (S Panel) and the FusionPlex ALK, RET, and ROS1 ver2 Panel (ARR Panel).
RESULTS
A total of 97 specimens were examined and 620 candidates were detected and categorized as WEFCs. A median of five WEFCs were detected per sample. In the S Panel group, there were 13 WEFCs with a frequency of more than 1%. In the ARR Panel group, a total of 16 WEFCs were detected with a frequency of more than 1%.
CONCLUSION
Specific WEFCs were detected according to the panel selected.
背景/目的：Archer FusionPlex 平台广泛用于癌症组织中综合融合基因检测。该平台分别显示强证据和弱证据融合候选物（WEFC）的结果。在弱证据类别中经常发现独特的融合模式，这些模式的信息在临床上至关重要。
患者和方法
我们描述了使用 FusionPlex 肉瘤Panel（S Panel）和 FusionPlex ALK、RET 和 ROS1 ver2 Panel（ARR Panel）观察到的 WEFC 类型和频率。
结果
共检查了 97 个标本，检测到并分类了 620 个候选物作为 WEFC。每个样本检测到的 WEFC 中位数为 5 个。在 S Panel 组中，有 13 个 WEFC 的频率超过 1%。在 ARR Panel 组中，共检测到 16 个 WEFC 的频率超过 1%。
结论
根据所选的面板检测到了特定的 WEFC。
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