Čanadi Jurešić Gordana, Ćurko-Cofek Božena, Barbarić Martina, Mumiši Nermina, Blagović Branka, Jamnik Polona
Department of Medical Chemistry, Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Rijeka, B. Branchetta 20, 51000, Rijeka, Croatia.
Department of Physiology and Immunology, Faculty of Medicine, University of Rijeka, B. Branchetta 20, 51000, Rijeka, Croatia.
Curr Microbiol. 2021 Apr;78(4):1188-1201. doi: 10.1007/s00284-021-02390-3. Epub 2021 Feb 23.
Yeast Saccharomyces cerevisiae is an ideal model organism for studying molecular mechanisms of the stress response provoked by metals. In this work, yeast cells response to iron (Fe) or lead (Pb) exposure was tested and compared. Survival test was used to determine testing doses of metal ions-for Fe it was 4 mM and for Pb 8 mM. These (high, over-loaded) doses provoked comparable values of growth inhibition, but different values in vitality measurement. The percentage of metabolically active cells, determined by fluorescent FUN-1 dye, was lower in Pb than in Fe treated cells. Besides, endogenous antioxidant defence systems in the cells treated with Pb were less efficient compared to Fe. At the mitochondrial level, the effects of metal ions were in correlation with the results of cell metabolic activity. The mitochondrial proteome of Pb treated cells showed the domination of protein downregulation. Yeast cells treated either with Fe or Pb shared 19 common significantly changed proteins. The affected proteins were involved in different cellular process and amongst them only five proteins belong to energy and carbohydrate metabolism, and protein biosynthesis. Based on all obtained results, it is possible to conclude that the effects of Fe and Pb on yeast cells show rather specific patterns of toxicity and stress response.
酿酒酵母是研究金属引发的应激反应分子机制的理想模式生物。在这项工作中，测试并比较了酵母细胞对铁（Fe）或铅（Pb）暴露的反应。采用存活试验来确定金属离子的测试剂量——铁为4 mM，铅为8 mM。这些（高、过载）剂量引发了相当的生长抑制值，但在活力测量中数值不同。用荧光FUN-1染料测定的代谢活性细胞百分比，铅处理的细胞低于铁处理的细胞。此外，与铁相比，铅处理的细胞内源性抗氧化防御系统效率较低。在线粒体水平上，金属离子的作用与细胞代谢活性结果相关。铅处理细胞的线粒体蛋白质组显示蛋白质下调占主导。用铁或铅处理的酵母细胞共有19种显著变化的共同蛋白质。受影响的蛋白质参与不同的细胞过程，其中只有五种蛋白质属于能量和碳水化合物代谢以及蛋白质生物合成。基于所有获得的结果，可以得出结论，铁和铅对酵母细胞的影响呈现出相当特定的毒性和应激反应模式。
