[MiR-600 suppresses HeLa cell proliferation by inhibiting hypoxia-inducible factor-1 signaling pathway].

OBJECTIVE

To determine whether miR-600 suppresses the proliferation of HeLa cells by inhibiting hypoxia-inducible factor-1 (HIF-1) signaling pathway and its effect on expressions of cyclin D1 and vascular endothelial growth factor (VEGF).

OBJECTIVE

HeLa cells were transfected with miR-600 mimic and plasmid-HIF-1, either alone or in combination, to up-regulate miR-600 and HIF-1 expressions in the cells. Six hours after the transfection, the cell viability was assessed using MTT assay, and the mRNA and protein expressions of VEGF, cyclin D1, and HIF-1 were analyzed with qPCR and Western blotting.

OBJECTIVE

The viability of HeLa cells showed no obvious changes 6 h after transfection with miR-600 mimic or Plasmid-HIF-1. At 24 h and 48 h, the cells transfected with miR-600 mimic showed a time-dependent reduction of cell viability, while the cells transfected with Plasmid-HIF-1 alone and with both miR-600 mimic and Plasmid-HIF-1 showed increased cell viability. The cell viabilities in Plasmid-HIF-1 group were significantly higher than those in miR-600 mimic+Plasmid-HIF-1 group at 24 h and 48 h. Six hours after transfection with miR-600 mimic, the cells exhibited significantly decreased expressions of VEGF, cyclin D1, and HIF-1, which were all significantly up-regulated in Plasmid-HIF-1 group and miR-600 mimic+Plasmid-HIF-1 group. VEGF, cyclin D1, and HIF-1 expressions were significant higher in Plasmid-HIF-1 group than in miR-600 mimic+ Plasmid-HIF-1 group.

OBJECTIVE

miR-600 suppresses the proliferation of HeLa cells and down-regulate the expressions of cyclin D1 and VEGF by inhibiting HIF-1 signaling pathway.