Suleyman Demirel University, Faculty of Dentistry, Department of Pediatric Dentistry, Isparta, Turkey.
Adnan Menderes University, Faculty of Dentistry, Department of Pediatric Dentistry, Aydın, Turkey.
J Appl Oral Sci. 2021 Feb 10;29:e20200414. doi: 10.1590/1678-7757-2020-0414. eCollection 2021.
The exposure to mercury (Hg) from dental amalgams is a suspected causative factor in neurological diseases. This study investigated the toxic effects of two different amalgam compositions related to Hg and the protective effects of selenium against the toxic effects of Hg through the TRPV1 channel in the human DBTRG glioblastoma cell line.
Six groups of the cells were organized. Analyses of cell viability, apoptosis, caspase 3 and caspase 9 activities, mitochondrial membrane depolarization, reactive oxygen species (ROS) production, and Western Blotting for protein expression levels were performed.
Cell viability values were lower in amalgam with high copper (HCu) and low copper (LCu) groups independently of time but were increased by selenium and capsazepine (p<0.001 and p<0.05). Conversely, apoptosis rates, caspase 3 and caspase 9 expression, ROS formation, mitochondrial membrane depolarization, and protein expression levels were higher in the HCu and LCu groups but were decreased by selenium (p<0.001 and p<0.05).
Selenium combined with an amalgam of either HCu or LCu decreases the toxic effects created by Hg in human DBTRG glioblastoma cells.
汞（Hg）暴露于汞合金被怀疑是神经系统疾病的一个致病因素。本研究通过人 DBTRG 神经胶质瘤细胞系中的 TRPV1 通道，调查了两种不同汞合金成分（与 Hg 相关）的毒性作用，以及硒对 Hg 毒性作用的保护作用。
将细胞分为六组。进行细胞活力、细胞凋亡、半胱天冬酶 3 和半胱天冬酶 9 活性、线粒体膜去极化、活性氧（ROS）产生以及 Western Blotting 分析蛋白表达水平的测定。
高铜（HCu）和低铜（LCu）组的细胞活力值在独立于时间的情况下均降低，但硒和辣椒素可使其增加（p<0.001 和 p<0.05）。相反，HCu 和 LCu 组的细胞凋亡率、半胱天冬酶 3 和半胱天冬酶 9 的表达、ROS 形成、线粒体膜去极化和蛋白表达水平均升高，但硒可降低这些指标（p<0.001 和 p<0.05）。
硒与 HCu 或 LCu 汞合金联合使用可降低 Hg 对人 DBTRG 神经胶质瘤细胞的毒性作用。
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