Department of Veterinary Microbiology and Infectious Diseases, Faculty of Veterinary Medicine, Vietnam National University of Agriculture, Hanoi, Vietnam.
Department of Veterinary Microbiology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Republic of Korea.
Vet Med Sci. 2021 Jul;7(4):1325-1331. doi: 10.1002/vms3.456. Epub 2021 Feb 24.
Swine abortion caused by viruses as well as bacteria has caused many economic losses in domestic farms over the years; however, bacterial abortion has not yet been studied in Korea. Several bacterial species were isolated from aborted fetuses (n = 103) for which the cause of death was not viral abortion. Among them, we focused on Aerococcus viridans, which had the highest positive rate within three provinces (Gangwon, Jeonnam and Gyeongnam). A total of 16 isolates were identified as A. viridans by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), and 13 were characterized by both antibiotic resistance and 16S rRNA gene analysis. Based on antibiotic susceptibility testing result, eight antimicrobials could not effectively eliminate the present isolation (more than 40% of isolates can resist these antibiotics), while all except two strains were susceptible to trimethoprim/sulfamethoxazole. Molecular analysis indicated genetic variation among these strains. This study is the first report detecting A. viridans from aborted fetuses in Korean domestic farms.
多年来，病毒和细菌引起的猪流产给国内养殖场造成了许多经济损失；然而，在韩国，尚未对细菌性流产进行研究。从 103 例死因非病毒性流产的流产胎儿中分离出了几种细菌。其中，我们重点研究了在三个省（江原道、全罗南道和庆尚南道）阳性率最高的微球菌属。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）鉴定，共鉴定出 16 株 A. viridans 为 A. viridans，其中 13 株通过抗生素耐药性和 16S rRNA 基因分析进行了特征描述。根据抗生素药敏试验结果，8 种抗菌药物不能有效消除本研究中的分离株（超过 40%的分离株对这些抗生素有耐药性），而除了两株之外的所有菌株均对甲氧苄啶/磺胺甲噁唑敏感。分子分析表明这些菌株存在遗传变异。本研究首次报道了从韩国国内养殖场流产胎儿中检测到 A. viridans。
