Suppr超能文献

对……叶绿体基因组进行测序和比较分析,为……的标记开发和系统发育学提供见解。

Sequencing and comparative analysis of the chloroplast genome of provide insights for marker development and phylogenetics in .

作者信息

Wang Le, Liang Jian, Sa Wei, Wang Li

机构信息

State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xi'ning, 810016 China.

Qinghai Academy of Agricultural Forestry Sciences, Qinghai University, Xi'ning, 810016 China.

出版信息

Physiol Mol Biol Plants. 2021 Jan;27(1):81-92. doi: 10.1007/s12298-021-00932-4. Epub 2021 Feb 1.

Abstract

UNLABELLED

commonly known as clove currant, is a perennial deciduous shrub noted for its long-lasting fragrant flowers and edible fruits. Owing to its ornamental values, this species has been widely used in city gardening and urban landscaping. Here, the complete cp genome of was assembled for the first time. The plastome is 157,152 bp in length, with a GC content of 38.2%. The cp genome featured a typical quadripartite structure, consisting of a pair of inverted repeat regions of 25,961 bp, separated by a large single copy region of 86,896 bp, and a small single copy region of 18,333 bp. A total of 131 genes were annotated in the plastome, including 86 protein coding genes, 37 tRNA genes, and 8 rRNA genes. 56 SSRs were identified, among which, 82.35% were located in the intergenic regions. A strong A/T bias in base composition was observed in these cpSSRs. In addition, 49 repeats of different sizes and types were also found in the plastome. Through comparison, seven divergence hotspots were identified between the cp genomes of and var. . Sequences of these divergent regions could be developed as potential markers for species delimitation in further studies. We re-investigated the relationship aomong 32 species through plastome-based phylogenywhich revealed that as a sister of var. Thus, our study provides genomic resources and valuable reference for marker development and phylogenomics in .

SUPPLEMENTARY INFORMATION

The online version of this article (10.1007/s12298-021-00932-4) contains supplementary material, which is available to authorized users.

摘要

未标注

通常被称为丁香醋栗,是一种多年生落叶灌木,以其持久的香花和可食用果实而闻名。由于其观赏价值,该物种已广泛应用于城市园艺和城市景观美化。在此,首次组装了该物种的完整叶绿体基因组。该质体基因组长度为157,152 bp,GC含量为38.2%。叶绿体基因组具有典型的四分体结构,由一对25,961 bp的反向重复区域组成,被一个86,896 bp的大单拷贝区域和一个18,333 bp的小单拷贝区域隔开。质体基因组中共注释了131个基因,包括86个蛋白质编码基因、37个tRNA基因和8个rRNA基因。鉴定出56个简单序列重复(SSR),其中82.35%位于基因间隔区。在这些叶绿体SSR中观察到碱基组成存在强烈的A/T偏向。此外,在质体基因组中还发现了49个不同大小和类型的重复序列。通过比较,在该物种和变种的叶绿体基因组之间鉴定出七个差异热点。这些差异区域的序列可在进一步研究中开发为物种界定的潜在标记。我们通过基于质体基因组的系统发育重新研究了32个该物种之间的关系,结果表明该物种是变种的姐妹种。因此,我们的研究为该物种的标记开发和系统发育基因组学提供了基因组资源和有价值的参考。

补充信息

本文的在线版本(10.1007/s12298-021-00932-4)包含补充材料,授权用户可获取。

相似文献

1
Sequencing and comparative analysis of the chloroplast genome of provide insights for marker development and phylogenetics in .
Physiol Mol Biol Plants. 2021 Jan;27(1):81-92. doi: 10.1007/s12298-021-00932-4. Epub 2021 Feb 1.
2
The complete plastome of Sorbaria kirilowii: genome structure, comparative analysis, and phylogenetic implications.
Mol Biol Rep. 2020 Dec;47(12):9677-9687. doi: 10.1007/s11033-020-05976-5. Epub 2020 Nov 7.
6
The complete plastome of (Lour.) DC. 1928 (Myrtaceae).
Mitochondrial DNA B Resour. 2022 Apr 25;7(4):705-706. doi: 10.1080/23802359.2022.2067015. eCollection 2022.
10
A complete chloroplast genome of a traditional Chinese medicine herb, , and phylogenomics of Rubiaceae.
Physiol Mol Biol Plants. 2023 Jun;29(6):843-853. doi: 10.1007/s12298-023-01302-y. Epub 2023 Jun 20.

本文引用的文献

2
Plastome Evolution in Saxifragaceae and Multiple Plastid Capture Events Involving and .
Front Plant Sci. 2020 Apr 24;11:361. doi: 10.3389/fpls.2020.00361. eCollection 2020.
3
Analysis of complete chloroplast genomes of Curcuma and the contribution to phylogeny and adaptive evolution.
Gene. 2020 Mar 30;732:144355. doi: 10.1016/j.gene.2020.144355. Epub 2020 Jan 11.
5
CPGAVAS2, an integrated plastome sequence annotator and analyzer.
Nucleic Acids Res. 2019 Jul 2;47(W1):W65-W73. doi: 10.1093/nar/gkz345.
8
Editorial: Structure and Function of Chloroplasts.
Front Plant Sci. 2018 Nov 14;9:1656. doi: 10.3389/fpls.2018.01656. eCollection 2018.
9
Practical considerations for plant phylogenomics.
Appl Plant Sci. 2018 Apr 2;6(3):e1038. doi: 10.1002/aps3.1038. eCollection 2018 Mar.
10
MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms.
Mol Biol Evol. 2018 Jun 1;35(6):1547-1549. doi: 10.1093/molbev/msy096.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验