Wang Le, Liang Jian, Sa Wei, Wang Li
State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xi'ning, 810016 China.
Qinghai Academy of Agricultural Forestry Sciences, Qinghai University, Xi'ning, 810016 China.
Physiol Mol Biol Plants. 2021 Jan;27(1):81-92. doi: 10.1007/s12298-021-00932-4. Epub 2021 Feb 1.
commonly known as clove currant, is a perennial deciduous shrub noted for its long-lasting fragrant flowers and edible fruits. Owing to its ornamental values, this species has been widely used in city gardening and urban landscaping. Here, the complete cp genome of was assembled for the first time. The plastome is 157,152 bp in length, with a GC content of 38.2%. The cp genome featured a typical quadripartite structure, consisting of a pair of inverted repeat regions of 25,961 bp, separated by a large single copy region of 86,896 bp, and a small single copy region of 18,333 bp. A total of 131 genes were annotated in the plastome, including 86 protein coding genes, 37 tRNA genes, and 8 rRNA genes. 56 SSRs were identified, among which, 82.35% were located in the intergenic regions. A strong A/T bias in base composition was observed in these cpSSRs. In addition, 49 repeats of different sizes and types were also found in the plastome. Through comparison, seven divergence hotspots were identified between the cp genomes of and var. . Sequences of these divergent regions could be developed as potential markers for species delimitation in further studies. We re-investigated the relationship aomong 32 species through plastome-based phylogenywhich revealed that as a sister of var. Thus, our study provides genomic resources and valuable reference for marker development and phylogenomics in .
The online version of this article (10.1007/s12298-021-00932-4) contains supplementary material, which is available to authorized users.
通常被称为丁香醋栗,是一种多年生落叶灌木,以其持久的香花和可食用果实而闻名。由于其观赏价值,该物种已广泛应用于城市园艺和城市景观美化。在此,首次组装了该物种的完整叶绿体基因组。该质体基因组长度为157,152 bp,GC含量为38.2%。叶绿体基因组具有典型的四分体结构,由一对25,961 bp的反向重复区域组成,被一个86,896 bp的大单拷贝区域和一个18,333 bp的小单拷贝区域隔开。质体基因组中共注释了131个基因,包括86个蛋白质编码基因、37个tRNA基因和8个rRNA基因。鉴定出56个简单序列重复(SSR),其中82.35%位于基因间隔区。在这些叶绿体SSR中观察到碱基组成存在强烈的A/T偏向。此外,在质体基因组中还发现了49个不同大小和类型的重复序列。通过比较,在该物种和变种的叶绿体基因组之间鉴定出七个差异热点。这些差异区域的序列可在进一步研究中开发为物种界定的潜在标记。我们通过基于质体基因组的系统发育重新研究了32个该物种之间的关系,结果表明该物种是变种的姐妹种。因此,我们的研究为该物种的标记开发和系统发育基因组学提供了基因组资源和有价值的参考。
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