Department of Orthopedics (Division of Hand Surgery), The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Key Laboratory of Orthopedics of Zhejiang Province, The Second School of Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, 325027, People's Republic of China.
Department of Post Anaesthesia Care Unit, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, The Second School of Medicine, Wenzhou Medical University, Wenzhou, 325027, Zhejiang, People's Republic of China.
Drug Des Devel Ther. 2021 Feb 18;15:679-688. doi: 10.2147/DDDT.S297458. eCollection 2021.
The application of random pattern skin flaps is limited in plastic surgery reconstruction due to necrosis. Trans-cinnamaldehyde has antibacterial, anticancer, and antioxidant properties. In this study, we aimed to investigate the effect of trans-cinnamaldehyde on skin flap survival and its possible mechanism regarding nitric oxide.
One hundred forty male Sprague-Dawley rats were randomly divided into seven groups (n = 20 each group). After the dorsal flap was raised, different doses of trans-cinnamaldehyde (10, 20, and 30 mg/kg) were immediately given by oral gavage in the three different groups. To assess the possible involvement of the nitric oxide system, N-nitro-L-arginine methyl ester (L-NAME, a nonselective nitric oxide synthase inhibitor) was used in this study. All flap samples were incised on postoperative day 7.
Our results showed that flap survival was increased significantly in the 20 mg/kg (P < 0.001) trans-cinnamaldehyde (TC) group compared to the control group or 30 mg/kg TC group. This protective function was restrained by coadministration of L-NAME with 20 mg/kg TC. The results of histopathology, laser Doppler, arteriography mediated with oxide-gelatine, and fluorescent staining all showed a significant increase in capillary count, collagen deposition, angiogenesis, and flap perfusion. Immunohistochemistry results revealed a significant increase in the expression of CD34, eNOS, and VEGF.
Trans-cinnamaldehyde increased flap survival through the nitric oxide synthase pathway and contributed to angiogenesis. A concentration of 20 mg/kg trans-cinnamaldehyde was recommended in this study.
由于坏死，随机皮瓣在整形重建中的应用受到限制。肉桂醛具有抗菌、抗癌和抗氧化特性。在这项研究中，我们旨在研究肉桂醛对皮瓣存活的影响及其与一氧化氮有关的可能机制。
140 只雄性 Sprague-Dawley 大鼠随机分为七组（每组 n = 20）。背部皮瓣抬起后，立即通过口服灌胃给予三种不同剂量的肉桂醛（10、20 和 30mg/kg）。为了评估一氧化氮系统的可能参与，本研究中使用了 N-硝基-L-精氨酸甲酯（L-NAME，一种非选择性一氧化氮合酶抑制剂）。所有皮瓣样本均在术后第 7 天切开。
我们的结果表明，与对照组或 30mg/kgTC 组相比，20mg/kgTC 组（P<0.001）皮瓣存活率显著增加。L-NAME 与 20mg/kgTC 联合给药可抑制这种保护作用。组织病理学、激光多普勒、氧化物-明胶介导的血管造影和荧光染色的结果均显示毛细血管计数、胶原蛋白沉积、血管生成和皮瓣灌注显著增加。免疫组织化学结果显示 CD34、eNOS 和 VEGF 的表达显著增加。
肉桂醛通过一氧化氮合酶途径增加皮瓣存活率，并有助于血管生成。在本研究中，建议使用 20mg/kg 肉桂醛。
