Pankovskiĭ D B, Stafeeva O A, Savitskiĭ A P
Probl Endokrinol (Mosk). 1988 Jan-Feb;34(1):57-60.
A method of synthesis of L-thyroxine-protein conjugates was described. It included an additional step of acylation of thyroxine by succinic anhydride. The acylated derivative was activated by carbodiimide to produce a compound which could react with the protein amino-groups. Protein conjugates with a high thyroxine content (over 20 thyroxine residues per molecule) were synthesized with minimal protein cross-linking using this method. The excess of acylated thyroxine was easily dialized against alkaline buffer. The rabbits immunized monthly with such conjugates gave antisera with high titers (1:2000-1:4000 by the ELISA method) in 9-13 weeks after the first injection. The method can be applied to other thyroid hormones and some-molecular-weight antigens.
描述了一种合成L-甲状腺素-蛋白质缀合物的方法。该方法包括用琥珀酸酐对甲状腺素进行酰化的额外步骤。酰化衍生物通过碳二亚胺活化以产生可与蛋白质氨基反应的化合物。使用该方法以最小的蛋白质交联合成了具有高甲状腺素含量（每分子超过20个甲状腺素残基）的蛋白质缀合物。过量的酰化甲状腺素很容易用碱性缓冲液透析。每月用此类缀合物免疫的兔子在首次注射后9-13周产生高滴度（通过ELISA法为1:2000-1:4000）的抗血清。该方法可应用于其他甲状腺激素和一些分子量的抗原。
