miRNA-140 对 SMMC-7721 肝癌细胞系生长及临床预后的影响。
Effects of miRNA-140 on the Growth and Clinical Prognosis of SMMC-7721 Hepatocellular Carcinoma Cell Line.
机构信息
Department of Microbiology, The School of Preclinical Medicine, Guangxi Medical University, Nanning, Guangxi 530021, China.
Department of Anatomy, Guangxi Medical University, 22 Shuangyong Road, Nanning, Guangxi 530021, China.
出版信息
Biomed Res Int. 2021 Feb 5;2021:6638915. doi: 10.1155/2021/6638915. eCollection 2021.
BACKGROUND
A growing number of studies have suggested that microRNAs exert an essential role in the development and occurrence of multiple tumours and act as crucial regulators in various biological processes. However, the expression and function of miRNA-140 in hepatocellular carcinoma (HCC) cells are not yet adequately identified and manifested.
METHODS
The expression of miRNA-140 was determined in HCC tissues and adjacent nontumour tissues by quantitative real-time polymerase chain reaction (qRT-PCR). Kaplan-Meier survival analysis and Cox regression analysis were performed to explore the correlation between miRNA-140 expression level and the survival rate of patients with HCC. Additionally, overexpression experiments were conducted to investigate the biological role of miRNA-140 in HCC cells. Bioinformatics was used to predict the related target genes and pathways of miRNA-140.
RESULTS
QRT-PCR results signified that the expression level of miRNA-140 in HCC was lower than that of adjacent normal tissues ( < 0.0001). Compared with the control group, the SMMC-7721 HCC cells in the miRNA-140 mimic group had a decrease in proliferation, migration, and invasion ( < 0.05), whereas those in the miRNA-140 inhibitor group had an increase in proliferation, migration, and invasion ( < 0.05). Cell cycle arrest occurred in the G0/1 phase. Prognosis analysis showed that the expression level of miRNA-140 was not related to the prognosis of HCC. Furthermore, the Kaplan-Meier test revealed that patients with lower miRNA-140 expression levels in liver cancer tissue had significantly shorter disease-free survival (DFS, = 0.004) and overall survival (OS) times ( = 0.010) after hepatectomy. Cox regression analysis further indicated that miRNA-140 was an independent risk factor that may affect the DFS ( = 0.004) and OS times ( = 0.014) of patients after hepatectomy. Our results suggested that miRNA-140 might be a crucial regulator involved in the HCC progression and is thus considered a potential prognostic biomarker and therapeutic target for HCC.
背景
越来越多的研究表明，微小 RNA （miRNA）在多种肿瘤的发生和发展中发挥着重要作用，并作为各种生物过程中的关键调节因子。然而，miRNA-140 在肝癌（HCC）细胞中的表达和功能尚未得到充分的鉴定和体现。
方法
通过实时定量聚合酶链反应（qRT-PCR）检测 HCC 组织和相邻非肿瘤组织中 miRNA-140 的表达。采用 Kaplan-Meier 生存分析和 Cox 回归分析探讨 miRNA-140 表达水平与 HCC 患者生存率的相关性。此外，进行过表达实验以研究 miRNA-140 在 HCC 细胞中的生物学作用。生物信息学用于预测 miRNA-140 的相关靶基因和通路。
结果
qRT-PCR 结果表明，miRNA-140 在 HCC 中的表达水平低于相邻正常组织（<0.0001）。与对照组相比，miRNA-140 模拟物组的 SMMC-7721 HCC 细胞增殖、迁移和侵袭能力降低（<0.05），而 miRNA-140 抑制剂组的增殖、迁移和侵袭能力增强（<0.05）。细胞周期阻滞发生在 G0/G1 期。预后分析表明，miRNA-140 的表达水平与 HCC 的预后无关。此外，Kaplan-Meier 检验显示，肝癌组织中 miRNA-140 表达水平较低的患者在肝切除术后无病生存（DFS）时间（=0.004）和总生存（OS）时间（=0.010）明显缩短。Cox 回归分析进一步表明，miRNA-140 是影响肝切除术后 DFS（=0.004）和 OS 时间（=0.014）的独立危险因素。我们的结果表明，miRNA-140 可能是 HCC 进展中的一个重要调节因子，因此被认为是 HCC 的一个潜在预后生物标志物和治疗靶点。
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