miR-193a-3p 通过调控 BTRC 促进胶质瘤的侵袭、迁移和间质转化。
miR-193a-3p Promotes the Invasion, Migration, and Mesenchymal Transition in Glioma through Regulating BTRC.
机构信息
Department of Pathology, School of Clinical Medicine, Weifang Medical University, Weifang, Shandong 261053, China.
Experimental Center for Medical Research, Weifang Medical University, Weifang, Shandong 261053, China.
出版信息
Biomed Res Int. 2021 Feb 9;2021:8928509. doi: 10.1155/2021/8928509. eCollection 2021.
BACKGROUND
The present study is aimed at exploring the specific expression of miR-193a-3p and the mechanism underlying miR-193a-3p-mediated mesenchymal transition (MT), invasion, and migration in glioma.
METHODS
The gene expression profile datasets of GSE39486 and GSE25676 were downloaded from the National Center for Biotechnology (NCBI). Data regarding the expression of miR-193a-3p and survival curves were derived from Chinese Glioma Genome Atlas (CGGA). Online websites including miRWalk, DIANA, and starbase were employed to predict the target genes for miR-193a-3p. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed by the Omicsbean online software. Module analysis of the protein-protein interaction (PPI) networks was performed by the plug-in Molecular Complex Detection (MCODE), and the degrees of genes were calculated by CytoHubba plug-in of Cytoscape. Survival curves were based on the Gene Expression Profile Interaction Analysis (GEPIA). Transwell, wound healing, and Western blot experiments were performed to investigate the effects of miR-193a-3p and beta-transducin repeat containing E3 ubiquitin protein ligase (BTRC) on the invasion, migration, and MT of glioma.
RESULTS
miR-193a-3p was highly expressed in glioma tissues and significantly correlated with poor survival in patients with glioma. The target genes for miR-193a-3p were involved in many cancer-related signaling pathways. The PPI showed 11 genes with both high degrees and MCODE scores in the network. Survival analysis demonstrated that the expression of BTRC was significantly correlated with the prognosis of patients with glioma. The results from the transwell, wound healing, and Western blot analyses suggested that miR-193a-3p promoted the invasion, migration, and MT of glioma cells, which could be reversed by BTRC.
CONCLUSIONS
miR-193a-3p was upregulated in patients with glioma and could affect the invasion, migration, and MT of glioma by regulating BTRC.
背景
本研究旨在探讨 miR-193a-3p 的特异性表达及其在胶质瘤中介导的间充质转化（MT）、侵袭和迁移的机制。
方法
从国家生物技术信息中心（NCBI）下载基因表达谱数据集 GSE39486 和 GSE25676。从中国脑胶质瘤基因组图谱（CGGA）获得 miR-193a-3p 的表达和生存曲线数据。利用 miRWalk、DIANA 和 starbase 等在线网站预测 miR-193a-3p 的靶基因。使用 Omicsbean 在线软件进行基因本体论（GO）和京都基因与基因组百科全书（KEGG）通路富集分析。通过 Cytoscape 的插件 Molecular Complex Detection（MCODE）进行蛋白质-蛋白质相互作用（PPI）网络的模块分析，通过 Cytoscape 的 CytoHubba 插件计算基因的度数。生存曲线基于基因表达谱交互分析（GEPIA）。通过 Transwell、划痕愈合和 Western blot 实验研究 miR-193a-3p 和β-转录重复含 E3 泛素蛋白连接酶（BTRC）对胶质瘤侵袭、迁移和 MT 的影响。
结果
miR-193a-3p 在胶质瘤组织中高表达，与胶质瘤患者的不良生存显著相关。miR-193a-3p 的靶基因参与许多癌症相关的信号通路。PPI 显示网络中存在 11 个具有高度数和 MCODE 分数的基因。生存分析表明，BTRC 的表达与胶质瘤患者的预后显著相关。Transwell、划痕愈合和 Western blot 分析结果表明，miR-193a-3p 促进了胶质瘤细胞的侵袭、迁移和 MT，而 BTRC 可以逆转这种作用。
结论
miR-193a-3p 在胶质瘤患者中上调，并可通过调节 BTRC 影响胶质瘤的侵袭、迁移和 MT。
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