Lee N S, Muhs G, Wagner G C, Reynolds R D, Fisher H
Department of Nutrition, State University of New Jersey, New Brunswick 08903.
Pharmacol Biochem Behav. 1988 Mar;29(3):559-64. doi: 10.1016/0091-3057(88)90020-2.
We studied the metabolic effects of high dietary intakes of pyridoxine and of the substrate-cofactor interaction between dietary histidine or tryptophan and pyridoxine in rat brain. In the substrate-cofactor interaction study, histamine and serotonin levels were determined in rats fed elevated or requirement levels of substrate (histidine: 0.3% and 0.8%, tryptophan: 0.15% and 0.6%) and excess or requirement levels of pyridoxine HCl (7 mg vs. 3,000 mg/kg). Excess pyridoxine intake caused a differential effect on brain histamine concentration--inhibitory with the requirement level of histidine (-29%), and stimulatory (+21%) with the elevated level of histidine. When dietary tryptophan was fed at the requirement level, excess pyridoxine caused essentially no changes in hypothalamic serotonin and 5HIAA (-2%, -2%). With elevated tryptophan intake, excess pyridoxine significantly increased serotonin and 5HIAA (+32%, +20%) in the hypothalamus. These results indicate a clear interaction between substrate and coenzyme precursor which influences brain metabolism of histamine and serotonin.
我们研究了大鼠脑中高膳食摄入量的吡哆醇以及膳食组氨酸或色氨酸与吡哆醇之间的底物-辅因子相互作用的代谢效应。在底物-辅因子相互作用研究中，测定了喂食底物（组氨酸：0.3%和0.8%，色氨酸：0.15%和0.6%）升高或需求水平以及过量或需求水平的盐酸吡哆醇（7毫克对3000毫克/千克）的大鼠体内组胺和血清素水平。过量摄入吡哆醇对脑组胺浓度产生了不同的影响——在组氨酸需求水平时具有抑制作用（-29%），而在组氨酸升高水平时具有刺激作用（+21%）。当膳食色氨酸以需求水平喂食时，过量的吡哆醇对下丘脑血清素和5-羟吲哚乙酸基本没有影响（-2%，-2%）。随着色氨酸摄入量的增加，过量的吡哆醇显著增加了下丘脑血清素和5-羟吲哚乙酸（+32%，+20%）。这些结果表明底物和辅酶前体之间存在明显的相互作用，这种相互作用影响了组胺和血清素的脑代谢。
