Berman R F, Crosland R D, Jenden D J, Altman H J
Department of Psychology, Wayne State University, Detroit, MI 48202.
Pharmacol Biochem Behav. 1988 Mar;29(3):581-6. doi: 10.1016/0091-3057(88)90023-8.
The effects of excitotoxic lesions of the nucleus basalis magnocellularis on cortical cholinergic activity and passive avoidance performance were examined in rats at 6, 14, 84 and 180 days after lesioning. Lesioned rats showed significant impairment of passive avoidance retention at every time point tested, with no evidence of behavioral recovery compared to unoperated and sham-lesioned (i.e., vehicle-injected) control rats. Cortical choline acetyltransferase (CAT) activity was reduced relative to controls at all time points examined, with the greatest reduction (i.e., 28%) occurring at approximately 14 days after lesioning. The levels of CAT activity at 180 postlesioning remained reduced compared to control animal levels, but less so than at 14 days after lesioning, indicating partial recovery. No changes in cholinergic muscarinic binding were observed at any time following lesioning. The results indicate that the behavioral and neurochemical effects of NbM lesions persist for at least 6 months following lesioning, but that partial, gradual recovery of cholinergic activity occurs.
在损毁基底大细胞核后6天、14天、84天和180天，检测了其对大鼠皮质胆碱能活性和被动回避行为表现的影响。在每个测试时间点，损毁大鼠的被动回避记忆均出现显著损伤，与未手术和假损毁（即注射溶剂）的对照大鼠相比，没有行为恢复的迹象。在所检测的所有时间点，皮质胆碱乙酰转移酶（CAT）活性相对于对照组均降低，在损毁后约14天时降低最为显著（即28%）。与对照动物水平相比，损毁后180天时CAT活性水平仍降低，但低于损毁后14天时，表明有部分恢复。损毁后任何时间均未观察到胆碱能毒蕈碱结合的变化。结果表明，基底大细胞核损毁的行为和神经化学效应在损毁后至少持续6个月，但胆碱能活性会出现部分、逐渐的恢复。
