Meltzer L T, Rosecrans J A
Department of Pharmacology and Toxicology, School of Basic Health Sciences, Virginia Commonwealth University, Richmond 23298.
Pharmacol Biochem Behav. 1988 Mar;29(3):587-93. doi: 10.1016/0091-3057(88)90024-x.
The cholinergic innervation of central muscarinic (M-Ch) and nicotinic (N-Ch) receptors was evaluated by studying the interaction of physostigmine with the discriminative stimulus (DS) effects of arecoline and nicotine. Rats were trained to discriminate either arecoline (1.74 mg/kg) or nicotine (1.14 mg/kg) from saline using a two-lever, milk reinforced, operant task. Physostigmine (0.125 mg/kg) pretreatment potentiated, and when administered alone (0.25 mg/kg), generalized with the DS induced by arecoline. In contrast, physostigmine, at the same dose, neither potentiated nor generalized with the DS effects of nicotine. These findings provide evidence that central muscarinic receptors are cholinergically innervated (physiologic) while central nicotinic receptors are not cholinergically innervated but are cholinoceptive (pharmacological).
通过研究毒扁豆碱与槟榔碱和尼古丁的辨别刺激（DS）效应之间的相互作用，评估了中枢毒蕈碱（M-Ch）和烟碱（N-Ch）受体的胆碱能神经支配。使用双杠杆、牛奶强化的操作性任务，训练大鼠从盐水中辨别槟榔碱（1.74 mg/kg）或尼古丁（1.14 mg/kg）。毒扁豆碱（0.125 mg/kg）预处理增强了槟榔碱诱导的DS效应，且单独给药（0.25 mg/kg）时，会产生与槟榔碱诱导的DS效应相同的泛化作用。相比之下，相同剂量的毒扁豆碱既不增强尼古丁的DS效应，也不会产生与尼古丁DS效应相同的泛化作用。这些发现提供了证据，表明中枢毒蕈碱受体接受胆碱能神经支配（生理性的），而中枢烟碱受体不接受胆碱能神经支配，但具有胆碱感受性（药理学性的）。
