Global Rust Reference Center, Department of Agroecology, Faculty of Science and Technology, Aarhus University, Forsøgsvej 1, 4200 Slagelse, Denmark.
IRTA, Institute for Food and Agricultural Research and Technology, 25198 Lleida, Spain.
Plant Dis. 2021 Sep;105(9):2281-2285. doi: 10.1094/PDIS-02-21-0269-SC. Epub 2021 Oct 28.
f. sp. , which causes yellow (or stripe) rust on wheat, is a macrocyclic and heteroecious fungus. In this study, we investigated whether subsp. and subsp. , which are indigenous in Spain, may serve as alternate hosts for f. sp. . Wheat leaves bearing telia of an isolate of f. sp. were harvested and used to inoculate plants of both barberry subspecies. Pycnia were observed on the adaxial side of the leaves from 10 days after inoculation (dai). Following successful fertilization, aecia were observed on the abaxial side of the leaves from 16 dai. At 27 dai, barberry leaves bearing aecia were detached and used to inoculate susceptible wheat seedlings of cultivar Morocco. Uredinia were observed on wheat seedlings from 12 days after aeciospore exposure. Eighty-three single lesions were recovered from individual wheat leaves, of which 43 were genotyped using 19 f. sp. simple sequence repeat markers (SSR). In total, 19 multilocus genotypes (MLGs) were identified among the 43 progeny isolates. The SSR genotyping confirmed that all 43 isolates were derived from the parental isolate. Seven heterozygous SSR markers showed segregation among the progenies, whereas none of the 12 homozygous markers resulted in segregation. These results demonstrated that subspp. and can serve as alternate hosts for f. sp. , which may result in novel virulence combinations that can have a detrimental impact on wheat production. Although f. sp. has not been detected on these barberry species in nature, this study highlights the importance of rust surveillance in barberry areas where suitable conditions for completion of the sexual life cycle may be present.
f. sp.，引起小麦黄花（或条锈）病，是一种大环和异宗真菌。在这项研究中，我们调查了是否原产于西班牙的 subsp. 和 subsp. 可能作为 f. sp. 的替代宿主。收获携带 f. sp. 厚垣孢子的 isolate 的小麦叶片，并将其用于接种两种小檗亚种的植物。在接种后 10 天（ dai ）观察到叶背侧的pycnia。成功受精后，在叶背侧观察到 aecia。在 27 dai 时，分离出带有 aecia 的小檗叶片并用于接种摩洛哥感病小麦幼苗。在小麦幼苗上从 aecospore 暴露后 12 天观察到 uredinia。从单个小麦叶片上恢复了 83 个单个病变，其中 43 个使用 19 个 f. sp. 简单序列重复标记（SSR）进行了基因型分析。在 43 个后代分离物中总共鉴定出 19 个多位点基因型（MLG）。SSR 基因型分析证实，所有 43 个分离物均源自亲本分离物。在后代中，有 7 个杂合 SSR 标记表现出分离，而 12 个纯合标记均未导致分离。这些结果表明，subpp. 和 可以作为 f. sp. 的替代宿主，这可能导致新的毒性组合，对小麦生产产生不利影响。尽管在自然界中尚未在这些小檗物种上检测到 f. sp.，但本研究强调了在可能存在有性生命周期完成的适宜条件的小檗地区进行锈病监测的重要性。
