利用表达β-葡萄糖苷酶的大肠杆菌将纤维二糖代谢工程化为 1,2-丙二醇的生产。
Metabolic engineering of 1,2-propanediol production from cellobiose using beta-glucosidase-expressing E. coli.
机构信息
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
Center for Sustainable Resource Science, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
出版信息
Bioresour Technol. 2021 Jun;329:124858. doi: 10.1016/j.biortech.2021.124858. Epub 2021 Feb 16.
Microbial 1,2-propanediol production using renewable feedstock is a promising method for the sustainable production of value-added fuels and chemicals. We demonstrated the metabolically engineered Escherichia coli for improvement of 1,2-propanediol production using glucose and cellobiose. The deletion of competing pathways improved 1,2-propanediol production. To reduce carbon flux toward downstream glycolysis, the phosphotransferase system (PTS) was inactivated by ptsG gene deletion. The resultant strain, GL3/PD, produced 1.48 ± 0.01 g/L of 1,2-propanediol from 20 g/L of glucose. A sugar supply was engineered by coexpression of β-glucosidase (BGL). The strain expressing BGL produced 1,2-propanediol from cellobiose at a concentration of 0.90 ± 0.11 g/L with a yield of 0.15 ± 0.01 g/g glucose (cellobiose 1 g is equal to glucose 1.1 g). As cellobiose or cellooligosaccharides a carbon source, the feasibility of producing 1,2-propanediol using an E. coli strain engineered for β-glucosidase expression are demonstrated.
利用可再生原料生产微生物 1,2-丙二醇是一种有前途的方法，可用于可持续生产增值燃料和化学品。我们展示了经过代谢工程改造的大肠杆菌，以提高利用葡萄糖和纤维二糖生产 1,2-丙二醇的能力。通过删除竞争途径来提高 1,2-丙二醇的产量。为了减少碳通量流向下游糖酵解途径，通过 ptsG 基因缺失使磷酸转移酶系统（PTS）失活。所得的 GL3/PD 菌株从 20 g/L 的葡萄糖中生产 1.48 ± 0.01 g/L 的 1,2-丙二醇。通过共表达β-葡萄糖苷酶（BGL）来设计糖源供应。表达 BGL 的菌株从纤维二糖生产 1,2-丙二醇，浓度为 0.90 ± 0.11 g/L，产率为 0.15 ± 0.01 g/g 葡萄糖（纤维二糖 1 g 等于葡萄糖 1.1 g）。作为纤维二糖或纤维寡糖等碳源，展示了通过表达β-葡萄糖苷酶工程改造的大肠杆菌菌株生产 1,2-丙二醇的可行性。
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