Koch G, Wiedemann K, Drebes E, Zimmermann W, Link G, Teschemacher H
Rudolf Buchheim-Institut für Pharmakologie, Justus Liebig-Universität, Giessen, F.R.G.
Regul Pept. 1988 Feb;20(2):107-17. doi: 10.1016/0167-0115(88)90044-4.
A method for the extraction of human beta-casomorphin-8 immunoreactive material from human plasma and a radioimmunoassay for its determination in the plasma extracts were developed. Blood was collected from 34 men, from 35 non-pregnant women, from 35 pregnant women and from 138 women after delivery and plasma extracts were assayed for the presence of human beta-casomorphin-8 immunoreactive materials. No human beta-casomorphin-8 immunoreactive material was detected in the plasma of men or non-pregnant women, whereas such material was found in the plasma of 26 out of 35 pregnant women and in the plasma of 100 out of 138 women after parturition. Material collected from women after delivery was characterized by gel filtration and high-performance liquid chromatography (HPLC) and was found to be of different composition in various individuals; its components, one of which coeluted with human beta-casein from the HPLC column, have apparently higher molecular weights than human beta-casomorphin-8. Some of these compounds seem to be very stable against enzymatic degradation at 37 degrees C in human plasma, whereas human beta-casomorphin-8 proved to be degraded very fast under identical conditions. A physiological significance of mammary products of the beta-casomorphin type during pregnancy or after parturition is suggested.
开发了一种从人血浆中提取人β-酪蛋白吗啡-8免疫反应性物质的方法以及用于测定血浆提取物中该物质的放射免疫分析法。从34名男性、35名未怀孕女性、35名孕妇和138名产后女性中采集血液，并对血浆提取物进行人β-酪蛋白吗啡-8免疫反应性物质检测。在男性或未怀孕女性的血浆中未检测到该物质，而在35名孕妇中的26名以及138名产后女性中的100名的血浆中发现了该物质。对产后女性采集的物质进行凝胶过滤和高效液相色谱（HPLC）分析，发现不同个体的该物质组成不同；其成分之一在HPLC柱上与人β-酪蛋白共洗脱，其分子量明显高于人β-酪蛋白吗啡-8。其中一些化合物在37℃的人血浆中似乎对酶降解非常稳定，而人β-酪蛋白吗啡-8在相同条件下被证明降解非常快。提示了β-酪蛋白吗啡型乳腺产物在孕期或产后的生理意义。
