Groux Raphaël, Stahl Elia, Gouhier-Darimont Caroline, Kerdaffrec Envel, Jimenez-Sandoval Pedro, Santiago Julia, Reymond Philippe
Department of Plant Molecular Biology, University of Lausanne, CH-1015 Lausanne, Switzerland.
Department of Biology, University of Fribourg, CH-1700 Fribourg, Switzerland.
Plant Physiol. 2021 Feb 25;185(1):240-255. doi: 10.1093/plphys/kiaa022.
In Arabidopsis (Arabidopsis thaliana), a hypersensitive-like response (HR-like response) is triggered underneath the eggs of the large white butterfly Pieris brassicae (P. brassicae), and this response is dependent on salicylic acid (SA) accumulation and signaling. Previous reports indicate that the clade I L-type LECTIN RECEPTOR KINASE-I.8 (LecRK-I.8) is involved in early steps of egg recognition. A genome-wide association study was used to better characterize the genetic structure of the HR-like response and discover loci that contribute to this response. We report here the identification of LecRK-I.1, a close homolog of LecRK-I.8, and show that two main haplotypes that explain part of the variation in HR-like response segregate among natural Arabidopsis accessions. Besides, signatures of balancing selection at this locus suggest that it may be ecologically important. Disruption of LecRK-I.1 results in decreased HR-like response and SA signaling, indicating that this protein is important for the observed responses. Furthermore, we provide evidence that LecRK-I.1 functions in the same signaling pathway as LecRK-I.8. Altogether, our results show that the response to eggs of P. brassicae is controlled by multiple LecRKs.
在拟南芥（Arabidopsis thaliana）中，粉纹夜蛾（Pieris brassicae）卵下方会触发类似超敏反应（HR样反应），且该反应依赖于水杨酸（SA）的积累和信号传导。先前的报道表明，I类L型凝集素受体激酶I.8（LecRK-I.8）参与了卵识别的早期步骤。为了更好地表征HR样反应的遗传结构并发现促成该反应的基因座，我们进行了全基因组关联研究。我们在此报告了LecRK-I.1的鉴定，它是LecRK-I.8的紧密同源物，并表明解释HR样反应部分变异的两种主要单倍型在天然拟南芥种质中分离。此外，该基因座的平衡选择特征表明它可能具有重要的生态意义。LecRK-I.1的破坏导致HR样反应和SA信号传导减弱，表明该蛋白对观察到的反应很重要。此外，我们提供证据表明LecRK-I.1与LecRK-I.8在同一信号通路中发挥作用。总之，我们的结果表明，对粉纹夜蛾卵的反应由多个LecRK控制。
