Center for Membrane and Cell Physiology, University of Virginia, Charlottesville, VA, USA.
Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA, USA.
Nat Methods. 2021 Mar;18(3):258-261. doi: 10.1038/s41592-021-01078-7. Epub 2021 Feb 25.
Serotonin (5-HT) is an important signaling monoamine and neurotransmitter. We report structure-guided engineering of a green fluorescent, genetically encoded serotonin sensor (G-GESS) from a 5-HT-binding lipocalin in the soft tick Argas monolakensis. G-GESS shows fast response kinetics and high affinity, specificity, brightness and photostability. We used G-GESS to image 5-HT dynamics in cultured cells, brain slices and behaving mice.
血清素（5-HT）是一种重要的信号单胺类神经递质。我们报道了一种来自软蜱 Argas monolakensis 的 5-HT 结合脂蛋白的绿色荧光、基因编码的血清素传感器（G-GESS）的结构导向工程。G-GESS 具有快速的响应动力学和高亲和力、特异性、亮度和光稳定性。我们使用 G-GESS 来描绘培养细胞、脑切片和行为小鼠中的 5-HT 动态。
