Juhász B, Jécsai J, Szegedi B
Arch Tierernahr. 1978 Dec;28(11-12):685-99. doi: 10.1080/17450397809421010.
In order to find out how short-time denutrition changes the concentration of some substances in the rumen fluid and the blood, tests with full-grown sheep were carried out. Fodder was withheld from sheep with inserted Jarrett fistulae for 48 hours after normal feeding. After 48 hours the animals were given concentrated fodder, after 52 hours exclusively hay. From the 72nd hour onwards the animals were provided with fodder as usual. Samples of the rumen fluid and blood samples were taken at the beginning of the test, after the last normal feeding and then in the 24th, 32nd, 48th, 52nd, 56th, 72nd and 96th hour. We could find out that, during the 48-hour denutrition, the pH-value of the rumen fluid turned alkaline and the concentrations of ammonia, volatile fatty acids and lactic acid decreased. The protein metabolism underwent a rapid change in the organism. The protein content of the blood plasma decreased, above all the albumin content, as well as the concentration of glycoproteins and volatile amino acids. Among the various amino acids, the concentration of glycine increased highly, that of alanine and valine just slightly. The concentration of most amino acids decreased or--of some of them remained the same. Among the paramters that are characteristic of lipid and carbohydrate metabolism, the total content of lipids and cholesterin decreased, and so did the concentration of blood sugar, lactic acid and pyruvic acid in the blood plasma. The results indicate that short-time denutrition has a considerable influence on the rumen fermentation and the intermediary metabolism of ruminants. The quickly arising lack of energy of ruminants slows down the protein synthesis and increases the glyconeogenesis from amino acids. The tissue is supplied with energy by the mobilisation of lipids.
为了弄清楚短期营养缺乏如何改变瘤胃液和血液中某些物质的浓度，对成年绵羊进行了试验。正常喂食后，对安装了贾勒特瘘管的绵羊禁食48小时。48小时后给动物喂食浓缩饲料，52小时后只喂干草。从第72小时起，动物按常规喂食。在试验开始时、最后一次正常喂食后，然后在第24、32、48、52、56、72和96小时采集瘤胃液样本和血液样本。我们发现，在48小时的营养缺乏期间，瘤胃液的pH值变为碱性，氨、挥发性脂肪酸和乳酸的浓度降低。机体中的蛋白质代谢发生了快速变化。血浆中的蛋白质含量下降，尤其是白蛋白含量，以及糖蛋白和挥发性氨基酸的浓度。在各种氨基酸中，甘氨酸的浓度大幅增加，丙氨酸和缬氨酸的浓度仅略有增加。大多数氨基酸的浓度下降，或者其中一些保持不变。在脂质和碳水化合物代谢的特征参数中，脂质和胆固醇的总含量下降，血浆中的血糖、乳酸和丙酮酸浓度也下降。结果表明，短期营养缺乏对反刍动物的瘤胃发酵和中间代谢有相当大的影响。反刍动物迅速出现的能量缺乏减缓了蛋白质合成，并增加了由氨基酸生成糖的过程。通过脂质的动员为组织提供能量。
