Department of Chemistry, Oregon State University, Corvallis, Oregon, 97331, USA.
Whitney Laboratory for Marine Bioscience, Department of Chemistry, University of Florida, St. Augustine, Florida, 32080, USA.
Chem Biodivers. 2021 Apr;18(4):e2100046. doi: 10.1002/cbdv.202100046. Epub 2021 Mar 24.
From arid, high desert soil samples collected near Bend, Oregon, 19 unique bacteria were isolated. Each strain was identified by 16S rRNA gene sequencing, and their organic extracts were tested for antibacterial and antiproliferative activities. Noteworthy, six extracts (30 %) exhibited strong inhibition resulting in less than 50 % cell proliferation in more than one cancer cell model, tested at 10 μg/mL. Principal component analysis (PCA) of LC/MS data revealed drastic differences in the metabolic profiles found in the organic extracts of these soil bacteria. In total, fourteen potent antibacterial and/or cytotoxic metabolites were isolated via bioactivity-guided fractionation, including two new natural products: a pyrazinone containing tetrapeptide and 7-methoxy-2,3-dimethyl-4H-chromen-4-one, as well as twelve known compounds: furanonaphthoquinone I, bafilomycin C1 and D, FD-594, oligomycin A, chloramphenicol, MY12-62A, rac-sclerone, isosclerone, tunicamycin VII, tunicamycin VIII, and (6S,16S)-anthrabenzoxocinone 1.264-C.
从俄勒冈州本德附近干旱的高沙漠土壤样本中分离出 19 种独特的细菌。每种菌株都通过 16S rRNA 基因测序进行鉴定，并测试了它们的有机提取物的抗菌和抗增殖活性。值得注意的是，在 10μg/mL 的浓度下，有 6 种提取物（30%）表现出强烈的抑制作用，导致一种以上的癌细胞模型的细胞增殖率低于 50%。基于 LC/MS 数据的主成分分析（PCA）揭示了这些土壤细菌的有机提取物中代谢谱存在明显差异。总共通过基于生物活性的分离，分离出了 14 种具有潜在抗菌和/或细胞毒性的代谢产物，包括两种新的天然产物：含有吡嗪酮的四肽和 7-甲氧基-2,3-二甲基-4H-色烯-4-酮，以及 12 种已知化合物：呋喃并萘醌 I、巴佛洛霉素 C1 和 D、FD-594、寡霉素 A、氯霉素、MY12-62A、rac-鲨烯、isosclerone、衣霉素 VII、衣霉素 VIII 和（6S,16S）-蒽苯并恶嗪酮 1.264-C。
