Serdyuk I N, Grenader A K
Eur J Biochem. 1977 Oct 3;79(2):495-504. doi: 10.1111/j.1432-1033.1977.tb11833.x.
From the analysis of the measured radii of gyration of the RNA (Rg = 6.6 +/- 0.3 nm) and protein (Rg = 10.2 +/- 0.5 nm) components of the 50-S subparticle of Escherichia coli ribosomes it is concluded that proteins containing a large amount of hydrodynamically bound water are located on the periphery of the tightly packed RNA. We found that the common features of the measured X-ray scattering curves of the E. coli 70-S ribosome, its 30-S and 50-S subparticles and wheat 80-S ribosomes in the region of scattering angles corresponding to scattering vectors mu from 1 to 5 nm-1 reflect features of the RNA compact packing. A hypothesis is proposed that the compact packing of RNA helices in the range of Bragg distances of 4.5--2.0 nm is a general structural feature of all ribosomal particles.
通过对大肠杆菌核糖体50-S亚颗粒的RNA（回转半径Rg = 6.6 +/- 0.3纳米）和蛋白质（Rg = 10.2 +/- 0.5纳米）成分的回转半径测量分析得出结论，含有大量流体动力学结合水的蛋白质位于紧密堆积的RNA的外围。我们发现，大肠杆菌70-S核糖体、其30-S和50-S亚颗粒以及小麦80-S核糖体在散射角对应于散射矢量μ从1至5纳米-1的区域中测量的X射线散射曲线的共同特征反映了RNA紧密堆积的特征。提出了一个假说，即RNA螺旋在4.5--2.0纳米布拉格距离范围内的紧密堆积是所有核糖体颗粒的一个普遍结构特征。
