Student Research Committee, Department of Molecular Medicine, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
Student Research Committee, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Gene. 2021 May 25;782:145525. doi: 10.1016/j.gene.2021.145525. Epub 2021 Feb 23.
There have been a few studies on the association between the angiopoietin-like 3 (ANGPTL3) single nucleotide polymorphisms (SNPs) and the risk of cardiovascular disease (CVD). But there is no consensus about the association of ANGPTL3 haplotypes and cardiometabolic disorders. We aimed to determine the association of three variants of the ANGPTL3 gene and CVD risk factors, which included: diabetes mellitus, hypertension, obesity, metabolic syndrome, and dyslipidaemia in the MASHAD population cohort.
DNA extraction and genotyping were undertaken in1002 individuals who were recruited as part of the Mashhad-Stroke and Heart-Atherosclerotic-Disorders (MASHAD) cohort. The association between the rs1748195, rs11207997, and rs10789117 variants with CVD event following 6 years follow-up and individual CVD risk factors were assessed using multivariate analysis.
Individuals with a GTC haplotype had a reduced risk of CVD, dyslipidaemia, obesity, and DM. Moreover, we found that of all 8 haplotypes, the CTC was associated with a 1.5-fold higher risk of HTN. Carriers of an uncommon allele of the ANGPTL3 gene had a lower risk of obesity, HTN, MetS and DM (rs10789117), and in those with the rs1748195 variant there was a lower risk of obesity compared to the wild-type genotype.
We found that the GTC and CTC haplotypes of the ANGPTL3 gene may help identify individuals with a genetic susceptibility to cardiometabolic disorders.
有几项关于血管生成素样 3（ANGPTL3）单核苷酸多态性（SNP）与心血管疾病（CVD）风险之间关联的研究。但是，ANGPTL3 单倍型与心脏代谢紊乱之间的关联尚无定论。我们旨在确定 ANGPLT3 基因的三个变体与 CVD 风险因素（包括糖尿病、高血压、肥胖、代谢综合征和血脂异常）之间的关联，这些因素在 MASHAD 人群队列中。
在 Mashhad-Stroke 和 Heart-Atherosclerotic-Disorders（MASHAD）队列中招募的 1002 名个体中进行 DNA 提取和基因分型。使用多元分析评估 rs1748195、rs11207997 和 rs10789117 变体与 6 年后 CVD 事件之间的关联以及个体 CVD 风险因素。
GTC 单倍型个体患 CVD、血脂异常、肥胖和 DM 的风险降低。此外，我们发现，在所有 8 种单倍型中，CTC 与 HTN 的风险增加 1.5 倍相关。ANGPTL3 基因罕见等位基因的携带者患肥胖症、HTN、代谢综合征和 DM（rs10789117）的风险较低，与野生型基因型相比，携带 rs1748195 变异体的个体患肥胖症的风险较低。
我们发现，ANGPTL3 基因的 GTC 和 CTC 单倍型可能有助于确定具有心脏代谢紊乱遗传易感性的个体。
