Liu Lingyuan, Zhang Qianyi, Li Feng, Wang Mei, Sun Jing, Zhu Shuyun
School of Chemistry and Chemical Engineering, Qufu Normal University, Qufu 273165, China.
Qingdao Special Service Men Recuperation Center of PLA Navy, Qingdao 266071, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 May 15;253:119584. doi: 10.1016/j.saa.2021.119584. Epub 2021 Feb 14.
Herein, fluorescent DNA-templated silver nanoclusters (DNA-AgNCs) with red emission were synthesized and utilized as novel probe to detect D-penicillamine (D-Pen) for the first time. D-Pen molecules contain a thiol which can combine with Ag to form a non-fluorescent ground state complex, inducing the aggregation of DNA-AgNCs followed by the fluorescence quenching. The quenching mechanism is well-studied and found to be a static quenching process. This method can detect D-Pen in the range of 0.025-0.7 μM with the detection limit as low as 8 nM, which is 1-3 orders of magnitude more sensitive than those based on other fluorescent nanoprobes. More importantly, the preparation procedure for DNA-AgNCs is fast and without the requirement of heavy metal ions. Thus, this detection strategy is time-saving and eco-friendly. Satisfactory recoveries have been acquired for monitoring D-Pen in human serum samples and pharmaceutical samples owing to the high sensitivity.
在此，合成了具有红色发射的荧光DNA模板银纳米簇（DNA-AgNCs），并首次将其用作新型探针检测D-青霉胺（D-Pen）。D-Pen分子含有一个硫醇，它可以与银结合形成非荧光基态复合物，诱导DNA-AgNCs聚集，随后荧光猝灭。对猝灭机制进行了深入研究，发现这是一个静态猝灭过程。该方法能够在0.025-0.7μM范围内检测D-Pen，检测限低至8 nM，比其他基于荧光纳米探针的方法灵敏1-3个数量级。更重要的是，DNA-AgNCs的制备过程快速，且无需重金属离子。因此，这种检测策略既节省时间又环保。由于具有高灵敏度，该方法用于监测人血清样品和药物样品中的D-Pen时获得了令人满意的回收率。
