一种基于无金属离子介导剂的DNA模板化银纳米簇的福美双检测荧光传感器。
A fluorescence sensor for thiram detection based on DNA-templated silver nanoclusters without metal ion-mediator.
作者信息
Yang Ziyan, Hu Lingling, Ning Keke, Wu Yuan, Liang Jiangong
机构信息
College of Science, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
College of Science, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
出版信息
Food Chem. 2023 Jul 1;413:135428. doi: 10.1016/j.foodchem.2023.135428. Epub 2023 Jan 7.
In this work, we firstly found a strong competitive interaction between thiram and silver atoms of DNA-templated silver nanoclusters (DNA-AgNCs), leading to fluorescence quenching of DNA-AgNCs without additional metal ion-mediator. Furthermore, this thiram-induced fluorescence quenching phenomenon was used to develop a sensor for thiram detection. This fluorescence sensor exhibited good linearity with thiram concentration from 0.20 to 2.0 μM and 0.012-0.20 μM under optimized conditions, with a low detection limit of 0.2 μM and 0.01 μM, respectively. Moreover, this sensor showed superior selectivity towards thiram, and its practicability was verified in apples and soil. This study provides a convenient and rapid "mix and detect" approach for thiram detection within 10 min, suggesting its potential for rapid on-site evaluation of thiram in real samples.
在本工作中，我们首次发现福美双与DNA模板化银纳米簇（DNA-AgNCs）中的银原子之间存在强烈的竞争相互作用，从而导致DNA-AgNCs的荧光猝灭，且无需额外的金属离子介导剂。此外，利用这种福美双诱导的荧光猝灭现象开发了一种用于检测福美双的传感器。在优化条件下，该荧光传感器在福美双浓度为0.20至2.0 μM和0.012 - 0.20 μM时表现出良好的线性关系，检测限分别低至0.2 μM和0.01 μM。此外，该传感器对福美双具有优异的选择性，并且在苹果和土壤中验证了其实用性。本研究提供了一种便捷快速的“混合检测”方法，可在10分钟内检测福美双，表明其在实际样品中对福美双进行快速现场评估的潜力。
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