Timoshenko A V, Zorin V P, Cherenkevich S N
Ukr Biokhim Zh (1978). 1988 Jan-Feb;60(1):41-6.
Fluorescein mercury acetate (FMA), a fluorescent probe, is used for the investigation of SH-groups of thymocytes' plasma membrane. It is found that mitogenic lectin Con A decreases the amount of membrane SH-groups and increases the fluorescence polarization degree of FMA (PFMA). The value of PFMA increases also during the incubation of cells with potassium ferricyanide and H2O2 but it decreases in the presence of NADH. The analysis of the data permits a conclusion that the thymocyte activation by Con A results in the selective oxidation of certain SH-groups with the formation of disulphide cross-linking between the plasma membrane receptors bound with the lectin molecules.
荧光素醋酸汞（FMA）是一种荧光探针，用于研究胸腺细胞质膜的巯基。发现促有丝分裂凝集素伴刀豆球蛋白A（Con A）会减少膜巯基的数量，并增加FMA的荧光偏振度（PFMA）。在用铁氰化钾和过氧化氢孵育细胞的过程中，PFMA的值也会增加，但在烟酰胺腺嘌呤二核苷酸（NADH）存在的情况下它会降低。对这些数据的分析得出一个结论，即Con A对胸腺细胞的激活导致某些巯基的选择性氧化，在与凝集素分子结合的质膜受体之间形成二硫键交联。
