鉴定 E2F 转录因子 7 作为口腔鳞状细胞癌的一种新的潜在生物标志物。
Identification of E2F transcription factor 7 as a novel potential biomarker for oral squamous cell carcinoma.
机构信息
Department of Stomatology, Jining No.1 People's Hospital, Jining, 272000, Shandong, China.
Department of Stomatology, Yantai Yuhuangding Hospital, Yantai, 264000, Shandong, China.
出版信息
Head Face Med. 2021 Feb 26;17(1):7. doi: 10.1186/s13005-021-00258-2.
BACKGROUND
As a tumor-accelerating transcriptional factor, E2F transcription factor 7 (E2F7) was up-regulated in many forms of cancers. Nevertheless, little has been reported about the impacts of E2F7 on oral squamous cell carcinoma (OSCC). Here, we aimed to probe whether E2F7 had influences on OSCC and its potential mechanism.
METHODS
The expression of E2F7 in OSCC tissues was analyzed using the data acquired from TCGA and ONCOMINE databases. E2F7 prognostic value in OSCC patients was analyzed utilizing TCGA database. The expression of E2F7 in OSCC cell lines was detected by qRT-PCR. Gain-and loss-function of E2F7 assays in TCA-83 and CAL27 cells were performed respectively to inquire the function of E2F7. Western blotting was applied to test the alternations of EMT-related markers.
RESULTS
In OSCC tissues, E2F7 was highly expressed. Besides, high expression of E2F7 predicted worse prognosis in OSCC patients. Moreover, E2F7 was over-expressed in TCA-83, HSC-4 and CAL27 (all OSCC cell lines) cells relative to that in HNOK (a normal cell line) cells. Gain-and loss-function assays displayed that deficiency of E2F7 suppresses CAL27 cell growth, migration, invasion and E2F7 high-expression resulted in inverse outcomes in TCA-83 cells. Finally, we found that silencing of E2F7 facilitated E-cadherin protein expression level and reduced N-cadherin, Vimentin and Snail protein levels in CAL27 cells, whilst E2F7 high-expression exhibited the opposite effects in TCA-83 cells.
CONCLUSIONS
These outcomes indicated that E2F7 performs a carcinogenic role in OSCC, which provides a theoretical basis for the therapeutic strategies of OSCC.
背景
作为一种肿瘤促进转录因子，E2F 转录因子 7（E2F7）在多种癌症中上调。然而，关于 E2F7 对口腔鳞状细胞癌（OSCC）的影响报道甚少。在这里，我们旨在探讨 E2F7 是否对 OSCC 有影响及其潜在机制。
方法
利用 TCGA 和 ONCOMINE 数据库获取的数据分析 OSCC 组织中 E2F7 的表达。利用 TCGA 数据库分析 E2F7 在 OSCC 患者中的预后价值。通过 qRT-PCR 检测 OSCC 细胞系中 E2F7 的表达。分别在 TCA-83 和 CAL27 细胞中进行 E2F7 的增益和缺失功能实验，以探究 E2F7 的功能。应用 Western blot 检测 EMT 相关标志物的变化。
结果
在 OSCC 组织中，E2F7 高表达。此外，E2F7 高表达预示着 OSCC 患者预后较差。此外，与 HNOK（正常细胞系）细胞相比，E2F7 在 TCA-83、HSC-4 和 CAL27（所有 OSCC 细胞系）细胞中过度表达。增益和缺失功能实验显示，E2F7 缺失抑制 CAL27 细胞的生长、迁移和侵袭，而 E2F7 高表达则导致 TCA-83 细胞的相反结果。最后，我们发现沉默 E2F7 促进了 CAL27 细胞中 E-钙粘蛋白蛋白表达水平，并降低了 N-钙粘蛋白、波形蛋白和 Snaill 蛋白水平，而 E2F7 高表达在 TCA-83 细胞中则表现出相反的作用。
结论
这些结果表明，E2F7 在 OSCC 中发挥致癌作用，为 OSCC 的治疗策略提供了理论依据。
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