Department of Stomatology, Tianjin Union Medical Center, Tianjin, 300121, People's Republic of China.
Department of Stomatology, Tianjin Union Medical Center, Tianjin, 300121, People's Republic of China.
Arch Oral Biol. 2021 May;125:105105. doi: 10.1016/j.archoralbio.2021.105105. Epub 2021 Mar 6.
The present study aimed to investigate the clinical significance and prognostic value of LINC01793 in OSCC patients, and to explore its role in the modulation of OSCC development.
LINC01793 expression was analyzed in 80 cases of OSCC patients and SCC9, SCC25, Cal27, and HN6 cell lines by qRT-PCR. The association of LINC01793 expression with clinicopathological features and prognosis in OSCC patients was analyzed. The effects of LINC01793 on cell proliferation, cell cycle, migration, and invasion of SCC9 and Cal27 cells were detected by MTT, flow cytometry, and Transwell assays in vitro, respectively.
LINC01793 level was upregulated in cancer tissues and cell lines of OSCC, and its expression was increased in cancer tissues from patients with lymph node metastasis. ROC curve for LINC01793 expression and lymph node metastasis revealed a significant AUC of 0.84 (95 % CI: 0.75-0.93), with 76.51 % sensitivity and 83.69 % specificity. Moreover, high LINC01793 level was positively correlated with T category, TNM stage, lymph node metastasis, and local recurrence. OSCC patients with high level of LINC01793 was followed by low overall survival rate, and LINC01793 expression was an independent prognostic indicator for overall survival in patients with OSCC. Functionally, cell proliferation, invasion and migration of SCC9 and Cal27 cells were decreased after knockdown of LINC01793. Consistently, silence of LINC01793 induced G0/G1 cell cycle arrest in OSCC cells.
High LINC01793 level is correlated with adverse clinicopathological features and poor prognosis of patients with OSCC. LINC01793 act as an oncogenic role in the development of OSCC.
本研究旨在探讨 LINC01793 在口腔鳞状细胞癌（OSCC）患者中的临床意义和预后价值，并探讨其在 OSCC 发展中的作用。
采用 qRT-PCR 分析 80 例 OSCC 患者和 SCC9、SCC25、Cal27 和 HN6 细胞系中 LINC01793 的表达。分析 LINC01793 表达与 OSCC 患者临床病理特征和预后的关系。分别采用 MTT、流式细胞术和 Transwell 实验检测 LINC01793 对 SCC9 和 Cal27 细胞增殖、细胞周期、迁移和侵袭的影响。
LINC01793 在癌症组织和 OSCC 细胞系中上调，且在有淋巴结转移的患者癌组织中表达增加。LINC01793 表达与淋巴结转移的 ROC 曲线分析显示，AUC 为 0.84（95 % CI：0.75-0.93），敏感性为 76.51 %，特异性为 83.69 %。此外，高 LINC01793 水平与 T 分期、TNM 分期、淋巴结转移和局部复发呈正相关。LINC01793 水平高的 OSCC 患者总生存率较低，LINC01793 表达是 OSCC 患者总生存率的独立预后指标。功能上，敲低 LINC01793 后 SCC9 和 Cal27 细胞的增殖、侵袭和迁移能力降低。同样，沉默 LINC01793 可诱导 OSCC 细胞 G0/G1 细胞周期停滞。
高 LINC01793 水平与 OSCC 患者不良临床病理特征和预后不良相关。LINC01793 在 OSCC 的发展中起致癌作用。
