Ring J, Hedin H, Richter W, Jesch F, Messmer K
Eur Surg Res. 1977;9(5):338-46. doi: 10.1159/000127953.
A high molecular weight component (HMC) of autolysate from Saccharomyces cerevisiae yeast cells was prepared. HMC was found to be immunogenic in dogs, inducing hemagglutinating antibody formation. Upon HMC challenge of immunized dogs, systemic anaphylactoid reactions were observed in 4/5 animals. The most prominent symptom was decreased cardiac output. Decrease in mean arterial pressure and increase in pulmonary arterial pressure were also observed. Consumption of total serum complement activity amounted to 22% of initial values. HMC also exhibited mitogenic activity in lymphocyte cultures from nonimmunized and immunized dogs. Since yeast autolysate is used as nitrogen source for Leuconostoc mesenteroides in the production of clinical B 512 dextran it is a theoretically possible trace contaminant of such solutions. Therefore, dogs hyperimmunized with HMC were also challenged with clinical dextran. No anaphylactoid signs were observed. These data suggest a negligible causal role of macromolecular contaminants derived from yeast cell autolysate in rare human anaphylactoid reactions following infusion of clinical dextran.
制备了来自酿酒酵母细胞自溶产物的一种高分子量成分（HMC）。发现HMC在犬体内具有免疫原性，可诱导血凝抗体形成。用HMC攻击免疫的犬后，在4/5的动物中观察到全身性类过敏反应。最突出的症状是心输出量降低。还观察到平均动脉压降低和肺动脉压升高。总血清补体活性消耗达初始值的22%。HMC在未免疫和免疫犬的淋巴细胞培养物中也表现出促有丝分裂活性。由于在临床B 512葡聚糖的生产中酵母自溶产物用作肠系膜明串珠菌的氮源，因此理论上它可能是此类溶液的一种微量污染物。因此，用HMC高度免疫的犬也用临床葡聚糖进行攻击。未观察到类过敏体征。这些数据表明，在输注临床葡聚糖后罕见的人类类过敏反应中，源自酵母细胞自溶产物的大分子污染物的因果作用可忽略不计。
