Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, China.
MoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Science, Huazhong University of Science and Technology, Wuhan, China.
J Biophotonics. 2021 Jun;14(6):e202000499. doi: 10.1002/jbio.202000499. Epub 2021 Mar 8.
In whole slide imaging (WSI), normally only a one layer imaging of the slide is performed. Autofocus at multiple positions is usually required. But defocus blur still exists due to tissue folding or specimen thickness. Repeated Z-stack scan be applied here, which, however, is too time consuming. Here, a high throughput slanted scanning WSI system is reported. In this system, the slide surface was slanted 1° relative to the focal plane. Thus, the focal plane spanned multiple layers of the sample. By moving the slide, multi-layer image data of the sample can be acquired simultaneously at a time frame comparable to conventional 1-layer imaging. With image fusion, defocus blur can be avoided. High quality and fast imaging of both cytological and histological slide specimens was demonstrated without applying aberration correction. The system can be a highly efficient way for the application of WSI in digital pathology.
在全玻片成像（WSI）中，通常仅对载玻片进行单层成像。通常需要在多个位置进行自动对焦。但是，由于组织折叠或标本厚度，仍然存在离焦模糊。可以在这里重复进行 Z 堆叠扫描，但是这太耗时了。在这里，报告了一种高通量倾斜扫描 WSI 系统。在该系统中，载玻片表面相对于焦平面倾斜 1°。因此，焦平面跨越了样本的多个层。通过移动载玻片，可以同时在与传统的单层成像相当的时间框架内获得样本的多层图像数据。通过图像融合，可以避免离焦模糊。在不应用像差校正的情况下，展示了对细胞学和组织学载玻片标本的高质量和快速成像。该系统可以成为数字病理学中 WSI 应用的高效方法。
