Department of Physiology, Tabriz Faculty of Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran.
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Horm Mol Biol Clin Investig. 2021 Feb 9;42(3):253-263. doi: 10.1515/hmbci-2020-0085.
High fat diet can lead to testicular structural and functional disturbances, spermatogenesis disorders as well as infertility. So, the present investigation was proposed to clarify whether voluntary exercise could prevent high fat diet induced reproductive complications in rats through testicular stress oxidative and apoptosis.
Forty male Wistar rats were randomly divided into four groups; control (C), voluntary exercise (VE), high fat diet (HFD) and high fat diet and voluntary exercise (VE + HFD) groups. The rats in the VE and VE + HFD groups were accommodated in apart cages that had running wheels and the running distance was assessed daily for 10 weeks. In VE + HFD group, animals were fed with HFD for five weeks before commencing exercise. The sperm parameters, the expressions of testicular miR-34a gene, and P53 and SIRT1 proteins as well as testicular apoptosis were analyzed in all groups.
The results indicated that voluntary exercise in VE + HFD group led to significantly increased GPX and SOD activities, SIRT1 protein expression, sperm parameters, and decreased the expression of miR34a gene and Acp53 protein, and cellular apoptosis index compared to HFD group (p<0.001 to p<0.05). The SOD and catalase activities, SIRT1 protein expression, sperm parameters in VE + HFD group were lower than of those of VE group, however, MDA content, expression of Acp53 protein, apoptosis indexes in VE + HFD group was higher than that of VE group (p<0.001 to p<0.05).
This study revealed that voluntary exercise improved spermatogenesis, in part by decreasing the testicular oxidative stress status, apoptosis through alteration in miR-34a/SIRT1/p53 pathway.
高脂肪饮食可导致睾丸结构和功能障碍、精子发生障碍以及不育。因此，本研究旨在阐明，通过睾丸应激氧化和细胞凋亡，自愿运动是否可以预防高脂肪饮食诱导的大鼠生殖并发症。
将 40 只雄性 Wistar 大鼠随机分为 4 组：对照组（C）、自愿运动组（VE）、高脂肪饮食组（HFD）和高脂肪饮食与自愿运动组（VE+HFD）。VE 和 VE+HFD 组的大鼠被安置在带有跑步轮的单独笼子中，每天评估跑步距离，持续 10 周。在 VE+HFD 组中，动物在开始运动前 5 周用 HFD 喂养。分析所有组的精子参数、睾丸 miR-34a 基因表达、P53 和 SIRT1 蛋白以及睾丸凋亡。
结果表明，与 HFD 组相比，VE+HFD 组中的自愿运动导致 GPX 和 SOD 活性、SIRT1 蛋白表达、精子参数显著增加，miR34a 基因表达和 Acp53 蛋白以及细胞凋亡指数降低（p<0.001 至 p<0.05）。VE+HFD 组的 SOD 和过氧化氢酶活性、SIRT1 蛋白表达、精子参数均低于 VE 组，但 VE+HFD 组的 MDA 含量、Acp53 蛋白表达、凋亡指数均高于 VE 组（p<0.001 至 p<0.05）。
本研究表明，自愿运动通过改变 miR-34a/SIRT1/p53 通路降低睾丸氧化应激状态和凋亡，从而改善精子发生。
