Rong Yawen, Ali Shujat, Ouyang Qin, Wang Li, Wang Bing, Chen Quansheng
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
College of Electrical and Electronic Engineering, Wenzhou University, Wenzhou 325035, PR China.
Food Chem. 2021 Jul 30;351:129215. doi: 10.1016/j.foodchem.2021.129215. Epub 2021 Feb 8.
This study describes a turn-on upconversion fluorescence sensor for the detection of acrylamide (AA) based on glutathione (GSH) modulated turn-on fluorescence strategy. Polyethyleneimine-modified upconversion nanoparticles were first prepared by the hydrothermal method and then Rhodamine B derivative (RBD) was loaded on their surface through non-covalent bonding. The GSH coupled with RBD and strongly quenched the upconversion fluorescence via fluorescence resonance energy transfer. Upon addition of tris (2-carboxyethyl) phosphine, the thiol-ene Michael addition reaction between GSH and AA was efficiently catalyzed, resulted in the quenched fluorescence triggered on. Under the optimum conditions, a linear detection range from 0.1 to 10 μM was implemented for AA with a limit of detection of 0.68 nM and great sensitivity was observed. Importantly, the proposed sensor was evaluated for spiked potato chips samples with a satisfactory result in contrast to high-performance liquid chromatography, confirmed its applicability for the rapid detection of AA.
本研究描述了一种基于谷胱甘肽（GSH）调控的开启荧光策略来检测丙烯酰胺（AA）的开启型上转换荧光传感器。首先通过水热法制备聚乙烯亚胺修饰的上转换纳米颗粒，然后通过非共价键合将罗丹明B衍生物（RBD）负载在其表面。GSH与RBD偶联并通过荧光共振能量转移强烈猝灭上转换荧光。加入三（2-羧乙基）膦后，有效催化了GSH与AA之间的硫醇-烯迈克尔加成反应，导致猝灭的荧光开启。在最佳条件下，对AA实现了0.1至10 μM的线性检测范围，检测限为0.68 nM，且具有很高的灵敏度。重要的是，与高效液相色谱法相比，对加标薯片样品评估了所提出的传感器，结果令人满意，证实了其在AA快速检测中的适用性。
