College of Food Science, Southwest University, Chongqing 400715, PR China.
College of Food Science, Southwest University, Chongqing 400715, PR China; Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, Chongqing 400715, PR China.
Food Chem. 2021 Jul 30;351:129286. doi: 10.1016/j.foodchem.2021.129286. Epub 2021 Feb 15.
Hidden mycotoxins have been reported to be "protected" by macromolecular substances to escape routine determination, but release to free mycotoxins under gastrointestinal conditions. Nowadays, the hidden zearalenone (ZEN) that binding with macromolecular zein has been found in maize. However, the binding mechanism of ZEN with zein in maize has not been clarified. In this study, the formation of ZEN-zein complex was investigated applying ultrafiltration, multi-spectroscopic and molecular modeling techniques. The steady-state and transient fluorescence analysis suggested the ZEN could interact with zein to form the complex driven by hydrophobic force and hydrogen bonds, which is in accordance with the molecular modeling studies. The conformational changes of zein induced by binding with ZEN were revealed by Fourier transform infrared spectroscopy (FTIR) and circular dichroism (CD). Elucidating the binding mechanism between zein and ZEN could help the development of detecting hidden ZEN and guarantee the safety of maize products.
据报道，隐藏的霉菌毒素被“保护”在高分子物质中，以逃避常规检测，但在胃肠道条件下会释放出游离的霉菌毒素。如今，已在玉米中发现与高分子醇溶蛋白结合的隐藏型玉米赤霉烯酮（ZEN）。然而，玉米中 ZEN 与醇溶蛋白的结合机制尚不清楚。本研究应用超滤、多光谱和分子建模技术研究了 ZEN-醇溶蛋白复合物的形成。稳态和瞬态荧光分析表明，ZEN 可以与醇溶蛋白相互作用形成复合物，驱动力为疏水作用力和氢键，这与分子建模研究结果一致。傅里叶变换红外光谱（FTIR）和圆二色性（CD）揭示了 ZEN 结合诱导醇溶蛋白构象的变化。阐明醇溶蛋白与 ZEN 之间的结合机制有助于开发检测隐藏型 ZEN 的方法，并保证玉米产品的安全。
