Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, School of Pharmacy, China Pharmaceutical University, No.24, Tongjiaxiang, Nanjing 210009, China; Department of Pharmaceutical Analysis, China Pharmaceutical University, No.24, Tongjiaxiang, Nanjing 210009, China.
Graduate School of Engineering, Osaka University, Osaka 565-0871, Japan.
J Chromatogr A. 2021 Mar 29;1641:462006. doi: 10.1016/j.chroma.2021.462006. Epub 2021 Feb 18.
Bio-based and low-cost hybrid polyvinyl alcohol (PVA) and gelatin (Gel) hydrophilic macromolecular complex coated microspheres were prepared based on one-pot process, characterized, and applied as novel sorbent materials for the purification of trace aminoglycosides from complex matrices. PVA acts as a "rigid" component in the hybrid complex to enhance its mechanical properties, while Gel's "flexible" role is to improve the swelling properties of the hybrid complex in water. It is shown that hybrid PVA/Gel-functionalized sorbents are more efficient than the respective PVA or Gel sorbents since the presence of Gel increases the material selectivity for aminoglycosides, which is due to the specific interactions occurring between the targets and amino acid residues in the hybrid materials. Under the optimum conditions, material preparation and pretreatment processes were entirely carried out in single water system without toxic organic solvent. The detection limit (LOD) of spectinomycin, kanamycin, streptomycin and dihydrostreptomycin in honey were 0.811, 0.303, 0.168, 0.045 μg⋅kg respectively. Linearity was obtained in the range of 20 to 2000 ug⋅kg, relative recovery yield up to 84.1-111.7% were obtained and matrix effect of all four aminoglycosides was within 100.8-107.6%. Intra-day and inter-day precision under four spiking levels (5, 200, 500 and 1000 ug⋅kg) were less than 10.9% (n=6) and 13.6% (n=3) respectively. In addition, the sorbents exhibited excellent reusability even after six recycles. This work demonstrates the potential of bio-based and low-cost hybrid polymer extraction platforms as promising bonded phase alternatives, in which eco-friendly and natural-based polymers can be used to improve the material selectivity and are conducive to the realization of "green chemistry".
基于一锅法制备了生物基、低成本的聚乙烯醇（PVA）和明胶（Gel）亲水性高分子复合微球，对其进行了表征，并将其作为新型吸附剂材料应用于从复杂基质中纯化痕量氨基糖苷类抗生素。PVA 在杂化复合物中充当“刚性”组分，以增强其机械性能，而 Gel 的“柔性”作用是提高杂化复合物在水中的溶胀性能。结果表明，与各自的 PVA 或 Gel 吸附剂相比，杂化 PVA/Gel 功能化吸附剂更为有效，因为 Gel 的存在增加了材料对氨基糖苷类的选择性，这是由于目标物与杂化材料中氨基酸残基之间发生了特定相互作用。在最佳条件下，材料的制备和预处理过程完全在单一水体系中进行，无需使用有毒有机溶剂。在蜂蜜中大观霉素、卡那霉素、链霉素和双氢链霉素的检测限（LOD）分别为 0.811、0.303、0.168 和 0.045μg·kg。在 20 至 2000μg·kg 的范围内得到了线性关系，相对回收率高达 84.1-111.7%，并且所有四种氨基糖苷类的基质效应均在 100.8-107.6%范围内。在四个加标水平（5、200、500 和 1000μg·kg）下，日内和日间精密度均小于 10.9%（n=6）和 13.6%（n=3）。此外，即使经过六次循环，该吸附剂仍表现出优异的可重复使用性。这项工作证明了生物基、低成本杂化聚合物萃取平台作为有前途的键合相替代品的潜力，其中可以使用环保和基于天然的聚合物来提高材料选择性，有利于实现“绿色化学”。
